| 紫外分光光度法测定维乐生片含量 |
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| 引用本文： | 李献洲 黎更豪. 紫外分光光度法测定维乐生片含量[J]. 中国医院药学杂志, 1994, 14(6): 267-269 |
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| 作者姓名： | 李献洲 黎更豪 |
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| 作者单位： | 广东省江门市人民医院 |
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| 摘 要： | 采用紫外分光光度法测定维乐生片中维生素Ｂ１和维生素Ｂ６含量，以磷酸盐缓冲液（ｐＨ７．０）作溶媒，制成溶液在３２５ｎｍ波长处测得的Ａ值为维生素Ｂ６的吸收度，吸收系数（Ｅ）为３５０；在２６８ｎｍ波长处测得的Ａ值减去Ａ３２５×０．２３２后的差值为维生素Ｂ１的吸收度，吸收系数（Ｅ）为２４５。回收率维生素Ｂ６为１００．２％，ＲＳＤ＝０．３％；维生素Ｂ１为１００．９％，ＲＳＤ＝０．９％。维生素Ｂ１２含量测定按广东省标准法。
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| 关 键 词： | 维乐生片，紫外分光光度法，含量测定 |
Ultraviolet spectrophotometric assay for weilasheng tablet |
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| Li Xian-zhou,Lin- Kang-ho. Ultraviolet spectrophotometric assay for weilasheng tablet[J]. Chinese Journal of Hospital Pharmacy, 1994, 14(6): 267-269 |
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| Authors: | Li Xian-zhou Lin- Kang-ho |
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| Abstract: | he contents of vitamine B1 and B6 in the weilasheng tablet was determined by ultraviolet spectrophotometry.Phosphatebuffer(pH 7.0)was used as solvent. The absorbance of vitamme B6 was measured at 325 nm,its E1 m was 350,the ab-sorbance of vitamine B1 was the difference of A268 and 0.232 A325,theE1%1 cm was 245. The recovery of vitamine B6 was100.2%RSD=0.3%.and of vitamine B1,was 100.9%,RSD=0.9%. |
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| Keywords: | weilasheng tablet ultroviolet spectrophotometry content assay |
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