ROS在长脉冲1064nm激光体外照射红色毛癣菌中的表达及作用

目的探讨活性氧簇(ROS)在长脉冲1 064 nm激光体外照射临床分离的红色毛癣菌Y和红色毛癣菌N中的表达变化及其作用。方法应用长脉冲Nd:YAG 1 064 nm激光分别以200 J/cm2,400 J/cm2、600 J/cm2能量照射红色毛癣菌Y菌落(分离自临床激光治疗有效的病甲)和红色毛癣菌N菌落(分离自临床激光治疗无效的病甲),未行激光照射组为对照组,观察红色毛癣菌Y和N的菌落在激光照射第1、3、5、7天的生长曲线变化,同时应用比色法检测ROS(H2O2、OH-、O2-)表达水平。结果红色毛癣菌Y随着激光照射能量的增加,生长曲线下移,在激光照射能量为600 J/cm2时,菌落停止生长,其ROS表达水平随照射剂量的增加而升高;红色毛癣菌N随着激光照射能量的增加,各激光能量组生长曲线接近重合,菌落生长不受影响,其ROS表达水平随照射剂量的增加无明显变化。结论 ROS可能在长脉冲1 064 nm激光治疗红色毛癣菌甲真菌病中发挥了作用。

Study on expression and role of reactive oxygen species in long pulse 1 064 nm laser irradiation on Trichophyton rubrum in vitro

Objective To explore the expression and role of reactive oxygen species（ ROS） in long pulse Nd： YAG 1 064 nm laser irradiation on isolates of Trichophyton rubrum Y（ T. rubrum Y） and Trichophyton rubrum N（ T. rubrum N）. Methods T. rubrum Y was isolated from onychomycosis patient with effective reaction on laser treatment and T. rubrum N was isolated from onychomycosis patient with bad reaction on laser treatment,and they were selected for this study. Each isolate with equal confluent colonies was irradiated in vitro by long pulse Nd： YAG 1064 nm laser with different energy（ 0 J /cm2,200 J/cm2,400 J/cm2,600 J/cm2） and 0 J/cm2group as control. Then the growth curve of each isolate after laser irradiation in day 1,3,5 and 7 had been observed. Furthermore,the level of ROS including hydrogenperoxide（ H2O2）,antihydroxyl radical（ anti- OH-）,anti- superoxide anion radical（ anti- O2-） of each T. rubrum isolate were detected by colorimetric method,respectively. Results The growth curve of isolate T. rubrum Y was moved down following the increase of laser energy. The growth of isolate T.rubrum Y had been stopped when laser energy was added up to 600 J /cm2. The level of ROS was positively correlated to laser energy. However,the growth curve of isolate T. rubrum N did not vary with the change of laser energy. The level of ROS was almost stable and it was not correlated to laser energy. Conclusion These findings suggest a possible role for ROS involving the inhibition on growth of T. rubrum during long pulse 1064 nm laser irradiation. ROS may play an important role on onychomycosis during laser treatment.