| 抗慢性B淋巴细胞白血病患者IgM单抗相对亲和力的分析 |
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| 引用本文： | 沈关心,苏娜,王晓林,朱慧芬,张悦,刘少祥. 抗慢性B淋巴细胞白血病患者IgM单抗相对亲和力的分析[J]. 细胞与分子免疫学杂志, 1992, 0(3) |
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| 作者姓名： | 沈关心 苏娜 王晓林 朱慧芬 张悦 刘少祥 |
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| 作者单位： | 同济医科大学实验医学研究中心免疫室，同济医科大学实验医学研究中心免疫室，同济医科大学实验医学研究中心免疫室，同济医科大学实验医学研究中心免疫室，同济医科大学实验医学研究中心免疫室，同济医科大学实验医学研究中心免疫室 武汉，430030，武汉，430030，武汉，430030，武汉，430030，武汉，430030，武汉，430030 |
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| 摘 要： | 本文采用间接ELISA法检测了11株抗B-CLL患者IgM腹水和杂交瘤上清McAb的相对亲和力。结果提示,各株McAb结合抗原的相对亲和力不同。根据50％最大结合浓度分为两组。其中4株杂交瘤上清McAb与纯化腹水McAb测得结果基本吻合。实验结果为合理地选用这些单抗提供了重要依据。
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| 关 键 词： | AcAb 相对亲和力 间接ELISA |
Determination of Relative Affinity of Monoclonal Anti-isotypic and Anti-idiotypic Antibodies against IgM from Chronic B Lymphocyte Leukaemia |
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| Shen Guanxin,Su Na,Wang Xiaolin,Zhu Huifen,Zhang Yue. Determination of Relative Affinity of Monoclonal Anti-isotypic and Anti-idiotypic Antibodies against IgM from Chronic B Lymphocyte Leukaemia[J]. Chinese journal of cellular and molecular immunology, 1992, 0(3) |
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| Authors: | Shen Guanxin Su Na Wang Xiaolin Zhu Huifen Zhang Yue |
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| Abstract: | In the present experiment, relative affinity was measured of 11 monoclonal antibodies (McAbs) against IgM from chronic B lymphocyte leukaemia using indirect ELISA. The results showed that the affinity of various McAbs to the same antigen was different and some of the difference was very evident. 11 McAbs could be divided into two groups by analyzing their 50% maximum binding. The relative affinity of McAbs in the culture supernatants was consistent with that of McAbs in the purified ascites. The study provides useful information for the rational application of these McAbs. |
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| Keywords: | relative affinity indirect ELISA antibody monoclonal |
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