子痫前期患者血浆对HUVEC凋亡和增殖的影响及其与LPA受体的关系

目的：探讨子痫前期（pre-eclampsia,PE）患者血浆对人脐静脉内皮细胞（HUVEC）凋亡和增殖的影响,并进一步分析其与溶血磷脂酸（lyso-phosphotidic acid,LPA）受体的关系,为其临床研究提供可参考依据。方法：收集2012年1月～2013年6月70例我院确诊的PE孕妇,其中轻度PE 38例,重度PE 32例,同时随机抽取30例正常孕妇作为对照组。所有孕妇空腹抽取静脉血,分别配成不同成分培养液对健康产妇分娩后的HUVEC进行培养,应用MTT比色法检测HUVEC的凋亡和增殖,采用免疫组化检测LPA受体Edg4、Edg7的表达。结果：重度PE组细胞增殖抑制率（IR）及LPA水平均高于轻度PE组及对照组,轻度PE组IR及LPA水平均高于对照组,差异均有统计学意义（P〈0.05）。PE孕妇HUVEC由长梭形变为圆（椭圆）形,细胞间隙增大,且细胞排列疏松,而重度PE组上述情况更明显。重度PE组Edg4阳性例数、mRNA表达及Edg7阳性例数、mRNA表达均高于轻度PE组及对照组,轻度PE组Edg4阳性例数、mRNA表达及Edg7阳性例数、mRNA表达均高于对照组,差异均有统计学意义（P〈0.05）。结论：内皮细胞损伤是PE发生的原因之一,且血浆LPA水平升高及其受体Edg4、Edg7表达增加可能参与了PE孕妇血管内皮细胞损伤的发生、发展过程。

Influence of plasma on proliferation and apoptosis of human umbilical vein endothelial cells and its correlation with lyso-phosphatidic acid

Objective：To investigate the influence of pre-eclampsia（PE）patients＇ plasma on proliferation and apoptosis of human umbilical vein endothelial cells（HUVEC）,ant to explore its correlation with lyso-phosphatidic acid（LPA）.Methods：A total of 38cases with the mild PE,32cases with the severe PE,30healthy pregnant women were observed.Plasma were collected from all subjects and HUVEC of healthy women were cultured.Cell proliferation and apoptosis was measured by MTT assay,and immunohistochemistry were used to measure the protein and mRNA expressions of Edg4and Edg7.Results：IR and LPA level in severe PE group were higher than that in mild PE group and the control group,the difference were all statistical significance（P0.05）.IR and LPA level in PE group were higher than that in control group,the difference were all statistical significance（P0.05）.HUVEC in healthy pregnant women deformed,from long shuttle to the circle（ellipse）,intercellular space increased,and cells loosed arranged,which were more obvious in severe PE group.Number of Edg4positive cases,mRNA expression and number of Edg7positive cases,mRNA expression in severe PE group were higher than that in mild PE group and the control group,the difference were all statistical significance（P0.05）.Number of Edg4positive cases,mRNA expression and number of Edg7positive cases,mRNA expression in mild group were higher than the control group,the difference were all statistical significance（P0.05）.Conclusion：Endothelial cell injury is one of the reasons for the PE occurrence,elevated plasma LPA levels and increased expression of receptor Edg4and Edg7may be involved in the occurrence and development of endothelial cell damage process in pregnant women with PE.