| Hedgehog通路阻滞剂GANT61对胰腺癌细胞株的抑制作用 |
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| 引用本文： | 杨胜兰,向正国. Hedgehog通路阻滞剂GANT61对胰腺癌细胞株的抑制作用[J]. 海南医学院学报, 2014, 20(1): 16-19 |
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| 作者姓名： | 杨胜兰 向正国 |
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| 作者单位： | 杨胜兰 (江苏无锡解放军第101医院消化内科,江苏无锡,214000)； 向正国 (江苏无锡解放军第101医院消化内科,江苏无锡,214000)； |
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| 基金项目： | This,Work,is,Financially,Supported,by,Special,Foundation,for,Clinical,Studies,from,Committee,of,Medical,Journal,of,Chinese,Universities(项目编号：112210923)中国高校医学期刊临床专项资金项目(项目编号：112210923) |
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| 摘 要： | 目的：探讨Hedgehog信号通路特异性阻滞剂GANT61抑制本通路在胰腺癌细胞株中的表达,观察胰腺癌细胞株增殖、迁移等能力的改变,为胰腺癌的治疗提供基础实验数据。方法：采用多株胰腺癌细胞,半定量RT-PCR法从中检测出Hedgehog通路表达相对活跃的细胞株,采用不同浓度的GANT61处理细胞株后,MTT法检测细胞株增殖活力改变,细胞划痕实验检测细胞迁移能力改变。结果：采用GANT61处理后,随着浓度的增加,胰腺癌细胞株增殖活力下降,迁移能力降低。结论：GANT61可通过抑制Hedgehog信号通路的表达,有效抑制胰腺癌细胞株的增殖、迁移等,为GNAT61药物在临床治疗胰腺癌提供扎实的理论基础。
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| 关 键 词： | 胰腺癌 Hedgehog通路 GANT61 |
GANT61 inhibited pancreatic cancer cell line by blocking Hedgehog signaling pathway |
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| YANG Sheng-lan;XIANG Zheng-guo. GANT61 inhibited pancreatic cancer cell line by blocking Hedgehog signaling pathway[J]. Journal of Hainan Medical College, 2014, 20(1): 16-19 |
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| Authors: | YANG Sheng-lan XIANG Zheng-guo |
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| Affiliation: | YANG Sheng-lan;XIANG Zheng-guo;Department of Gastroenterology No.101 Hospital of People’s Liberation Army; |
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| Abstract: | Objective：To explore the effects of GANT61inhibiting Hedgehog signaling pathway on proliferation and migration of human pancreatic cancer cells.Methods：Selected one cell line which has the relatively high activation in Hedgehog signaling pathway from four pancreatic tumor cell lines by semiquantity RT-PCR,detected cell proliferation viability by MTT and cell migration ability by cell scarification experiment after treatment of GANT61at different concentrations.Results：With the increase of GANT61concentration,the proliferation activity was decreased and migration ability was reduced in the pancreatic tumor cell.Conclusion：GANT61can effectively inhibit the proliferation and migration of pancreatic cancer cell lines by restricting Hedgehog signaling pathway. |
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| Keywords: | Pancreatic cancer Hedgehog signaling pathway GANT61 |
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