涂阳肺结核患者痰液胰酶预处理消毒效果分析

目的：研究肺结核患者痰液胰酶预处理后使用含氯消毒剂消毒的效果。方法将门诊收集116份痰涂片阳性肺结核患者标本随机分为试验组和对照组，试验组58例，以5%胰酶消化剂对涂阳患者不同性状痰液进行液化后，加入配置成含5000 mg/L有效氯的84消毒液进行消毒处理。对照组58例，痰液直接加入含5000 mg/L有效氯的84消毒液消毒。消毒剂作用0.5 h、1 h、2 h后，分别接种于中性罗氏培养基，对痰液中结核杆菌培养阳性情况进行比较分析。结果对照组84消毒液作用0.5 h、1 h、2 h后结核杆菌培养阳性率分别为100%、88%、79%；试验组经胰酶预处理后，再经84消毒液作用0.5 h、1 h、2 h后结核杆菌培养阳性率分别为62%、14%、0。结论涂阳肺结核患者痰液经胰酶液化预处理后，能促进消毒剂对痰液中结核杆菌的杀灭作用。在肺结核患者痰液的消毒中应积极推广痰液胰酶预处理消毒方法。

Study on the disinfection of pretreatment of pancreatic enzyme on pulmonary tuberculosis patients with sputum smear-positive

Objective To evaluate the effects of chlorine-containing disinfectant on TB sputum preprocessed by pancreatic enzyme. Methods 116 samples from sputum smear-positive pulmonary tuberculosis patients collected from outpatient service were randomly divided into the control group and treatment group. 58 samples of TB sputum in treatment group were preprocessed by 5%pancreatic enzyme disinfector, then to 84 disinfector of containing 5 000 mg/L chlorine. The 58 samples in control group, were directly processed by 84 disinfector of containing 5 000 mg/L chlorine. After 0.5 h, 1 h and 2 h of the treatment, the positive rates of mycobacterium tuberculosis of each sputum samples were comparatively analyzed. Results The positive rates of mycobacterium tuberculosis of 0.5 h, 1 h and 2 h in control group were 100%, 88%and 100%respectively. After pretreatment of pancreatic enzyme, the positive rates of 0.5 h, 1 h and 2 h in treatment group were 62%, 14%and 0 respectively.Conclusion The pretreatment of pancreatic enzyme on sputum of pulmonary tuberculosis patients can obviously promote the disinfectant effects. It should be actively promoted in disinfectants on TB sputum.