ELISA法检测中期因子在卵巢上皮性肿瘤中的表达及与其临床病理特征的关系

目的探讨中期因子（midkine，MK）在卵巢上皮肿瘤中的表达及与其临床病理特征的关系。方法采用双抗体夹心法（ELISA）检测50例上皮性卵巢癌、50例良性上皮性卵巢肿瘤及40例正常女性血清MK浓度。结果卵巢癌组血清MK含量明显高于卵巢良性肿瘤组与正常对照组的血清MK含量（P〈0．05），而卵巢良性肿瘤组与正常对照组的血清MK含量接近，差异无统计学意义（P〉0．05）；随着临床分期的提高，血清MK值逐渐增高；MK表达与肿瘤病理类型无相关性（P〉0．05）；随着病理分级的提高．血清MK值逐渐增高。结论卵巢癌患者血清MK含量明显高于卵巢良性肿瘤和正常人群，并与TNM分期和病理分级成正相关，MK可作为1种有价值的肿瘤标志物。

The Expression of Midkine in Epithelial Ovarian Tumor Detected by ELISA and Its Relationship with Clinicopathological Characteristics

Objective To investigate the expression of midkine （MK） in epithelial ovarian tumor and its relationship with clinico- pathological characteristics. Methods The double antibody sandwich enzyme-linked immunosorbent assay （ELISA） was used to measure MK levels in 50 patients with epithelial ovarian carcinoma, 50 cases of epithelial ovarian carcinoid and 40 normal women. Results The serum MK concentrations in ovarian carcinoma group were significantly higher than those of ovarian carcinoid group and those of normal control group （P〈0.05）, while serum MK concentrations of the ovarian carcinoid group were close to those of normal control group with no significant difference （P〉0.05）. The serum MK levels increased gradually with the clinical staging while there was no correlation between the MK expression and the tumor pathological types （P〉0.05）; The serum MK levels in- creased gradually with the increasing of pathological staging. Conclusion The serum MK contents in ovarian carcinoma patients were higher than those of ovarian carcinoid and normal ones, and which were positively correlated with TNM staging and patholog- ical grading. The MK could be used as a valuable tumor marker.