舍曲林对心梗后抑郁大鼠行为学及海马NR1表达的影响

目的:研究心梗后抑郁大鼠行为学及海马NR1蛋白表达的变化,以及舍曲林的干预作用。方法:将80只SD大鼠随机分为5组:正常对照组(n=10),心梗组(n=20),抑郁组(n=10),模型组(n=20),舍曲林组(n=20)。通过结扎冠状动脉前降支建立心肌梗死模型,予慢性不可预见性温和刺激建立抑郁模型,通过糖水消耗试验及旷场试验对动物进行行为学评分,采用Western blot方法对海马组织NR1的表达进行定量检测。结果:①与正常组相比,心梗组、抑郁组及模型组行为学评分下降:糖水偏爱百分比下降,运动减少,直立次数减少。②与正常组相比,心梗组、抑郁组及模型组海马NR1的表达量分别下降了12%±5%、30%±11%和57%±9%;舍曲林干预可增加模型组海马NR1表达(17%±6%)。结论:舍曲林可能通过调节海马NR1表达,改善心梗后抑郁大鼠抑郁样行为。

Effect of Sertraline on Behavior and Expression of NR1 in Hippocampus of Post-Myocardial Infarction Depression Rats

Objective:To observe the effect of sertraline on behavior in vivo in a rat model of post-MI depression,and to investigate the underlying molecular mechanism.Methods:Eighty SpragueDawley rats(150-200g)were randomly assigned to five groups:normal control rats(control), myocardial ischemia rats(MI),depression rats developed by chronic unpredictable mild stress(CUMS),myocardial ischemia rats with CUMS(model),and myocardial ischemia rats with CUMS treated with intragastric administration of 5mg/kg sertraline(sertraline).Behavior measurement was done before and during experiment.Electrophysiology study in vivo and molecular biological detection were carried on after 4-week CUMS.Results:After 4-week CUMS,depression-like behaviors were observed in CUMS and model groups,and these behaviors were relieved significantly after sertraline administration(P<0.05).Expression of NR1in hippocampus was reduced by 12±5%,30%±11%,and 57%±9%respectively in MI group,CUMS group,and model group,which could be increased by sertraline(17%±6%).Conclusion:Sertraline can increase the expression of NR1in hippocampus and improve depression-like behaviors in post-myocardial infarction depression rats.