大肠癌DAPK、FHIT基因启动子区的甲基化水平研究

目的:评价DAPK、FHIT基因启动子区甲基化状态在大肠癌发生发展中的作用和临床意义。方法:采用甲基化特异性聚合酶链反应(methylation-specific PCR,MSP)检测23例大肠癌及相应癌旁正常组织的DAPK、FHIT基因启动子区甲基化状态。结果:大肠癌组织的DAPK、FHIT基因甲基化率分别为60.86%(14/23)、52.17%(12/23),高于癌旁正常组织的13.04%(3/23)、8.69%(2/23),组间差异均有统计学意义(P<0.05),DAPK、FHIT基因甲基化状态与分化程度、淋巴结转移有显著相关性(P<0.05),与性别、年龄、肿瘤部位、Dukes分期无相关性(P>0.05)。结论:DAPK基因和FHIT基因启动子区的高甲基化可能与大肠癌的发生、发展、预后有关。

Methylation analysis of DAPK and FHIT gene promoter region in colorectal cancers

Objective： To evaluate the role and clinical significance of the methylation of the deathassociated protein kinase （DAPK） gene and fragile histidine triad （FHIT） in colorectal cancer. Methods： Methylation-specific PCR （MSP） was used to test methylation patterns of DAPK and FHIT gene in 23 colorectal cancer tissues and corresponding adjacent normal tissues. Results： Ab- errant methylation rates of DAPK and FHIT were detected in colorectal cancers （60.86%, 52.17% ） , which were significant higher than that in adjacent normal tissues（ 13.04% , 8.69% ）. The methylation status of DAPK and FHIT gene was associated with differentiated degree and lymph node metastasis （P 〈 0.05） , but not associated with age, gender, tumor site and Dukes stage （P 〉 0.05）. Conclusion ： The aberrant promoter methylation of DAPK and FHIT may be related with occurrence, development and clinical prognosis of colorectal cancer.