| Study of apoptosis induced by nordihydroguaiaretic acid in human ma-lignant glioma cell line SHG-44 |
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| 作者姓名： | 郭德玉 陈意生 卞修武 史景泉 陈自强 |
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| 作者单位： | InstituteofPathology,SouthwestHospital,ThirdMilitaryMedicalUniversity,Chongqing400038,China |
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| 基金项目： | National Natural Science Foundation of China (No. 39670296) |
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| 摘 要： | Objective: To investigate the effect and mechanism of nordihydroguaiaretic acid (NDGA) on apop-tosis in human malignant glioma cell line SHG-44. Methods: Cell growth inhibition was measured with MTT assay. Cell apoptosis was observed with light and electron microscopy and TUNEL. Expression of bcl-2 gene was measured with immunohistochemistry, in situ hybridization and image analyses. Results: NDGA at the concentration of 100 μmol/L inhibited the proliferation of SHG-44 cells and induced apoptosis in a time-de-pendent manner. The expression of Bcl-2 protein in SHG-44 cells was decreased in the present of 100 μmol/L NDGA along with the duration of treatment in a negative correlation with the degree of cell apoptosis. The bcl-2 mRNA expressed in SHG-44 cells was reduced after treatment with 100 μmol/L NDGA, apparently consistent with the immunohistochemical results. Conclusion.- NDGA can induce apoptosis of human malig-nant glioma cells probably by down-regulating expression of bcl-2 gene, though the exact mechanism needs further study.
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| 关 键 词： | 细胞凋亡 bcl-2 基因表达 SHG-44 神经胶质细胞 正二氢愈创酸 |
Study of apoptosis induced by nordihydroguaiaretic acid in human malignant glioma cell line SHG-44 |
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| GUO De-yu,CHEN Yi-sheng,BIAN Xiu-wu,SHI Jing-quan,CHEN Zi-qiang.Study of apoptosis induced by nordihydroguaiaretic acid in human ma-lignant glioma cell line SHG-44[J].Journal of Medical Colleges of PLA(China),2002,17(4):247-250,259. |
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| Authors: | GUO De-yu CHEN Yi-sheng BIAN Xiu-wu SHI Jing-quan CHEN Zi-qiang |
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| Abstract: | Objective:To investigate the effect and mechanism of nordihydroguaiaretic acid (NDGA) on apoptosis in human malignant glioma cell line SHG-44. Methods: Cell growth inhibition was measured with MTT assay. Cell apoptosis was observed with light and electron microscopy and TUNEL. Expression of bcl-2 gene was measured with immunohistochemistry, in situ hybridization and image analyses. Results: NDGA at the concentration of 100 μmol/L inhibited the proliferation of SHG-44 cells and induced apoptosis in a time-dependent manner. The expression of Bcl-2 protein in SHG-44 cells was decreased in the present of 100 μmol/L NDGA along with the duration of treatment in a negative correlation with the degree of cell apoptosis. The bcl-2 mRNA expressed in SHG-44 cells was reduced after treatment with 100 μmol/L NDGA, apparently consistent with the immunohistochemical results. Conclusion: NDGA can induce apoptosis of human malignant glioma cells probably by down-regulating expression of bcl-2 gene, though the exact mechanism needs further study. |
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| Keywords: | glioma nordihydroguaiaretic acid apoptosis bcl-2 |
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