一种胚胎移植新方法的建立

在转基因动物制作中胚胎移植是一个关键性技术。胚胎的输卵管移植相比子宫移植来说，可以避免在体外较长时间培养，但从输卵管伞部移植卵又常常不可避免地因囊膜开口而不同程度的出血，而且由于伞口部位置较隐蔽，移卵时技术难度大要求高。因此．我们尝试在囊膜外靠近壶腹部附近开口于输卵管，将卵直接移入壶腹部，卵移行至子宫发育。此技术非常简单易行，有助于提高转基因动物的制作效率。

A New Approach of Embryo Transfer by Oviduct

Following the microinjection procedure the eggs must be transferred to pseudopregnant recipient mothers. The oviduct transfer is preferably performed on the same days as the microinjections and it can minimize the time in culture compared with uterus transfer. Ordinary oviduct transfer is not easy because the infundibulium is hidden with the coils of oviduct, and the bursa must be torn and it frequently causes breeding. So, we directly make oviduct incision outside of the bursa just near the ampulla and eggs are transferred into ampulla. The new approach is easy because it can avoid breeding and the swollen ampulla is used as a good marker. This new approach will impove the efficiency of embryo transfer.