| 反相高效液相色谱法测定双黄连胶囊中绿原酸的含量 |
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| 引用本文： | 陈继英,赵辉,单振顺. 反相高效液相色谱法测定双黄连胶囊中绿原酸的含量[J]. 辽宁中医杂志, 2004, 31(8): 688-689 |
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| 作者姓名： | 陈继英 赵辉 单振顺 |
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| 作者单位： | 天津市大港医院,天津,300270;天津市大港医院,天津,300270;天津市大港医院,天津,300270 |
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| 摘 要： | 目的 :建立中成药双黄连胶囊中绿原酸的含量测定方法。方法 :采用Phenomenex -C1 8(2 5 0mm×4 6mm ,5 μm)柱 ,以水 -乙腈 -冰醋酸 (92∶7∶1 0 )为流动相 ,检测波长 32 6nm ,流速 1 0ml/min。结果 :绿原酸在 0 1 1～ 0 5 5 μg ,都与相应的峰面积呈良好的线性关系 (r=0 9999) ,平均回收率为 99 1 1 % ,RSD为 0 5 8% (n =6 )。结论 :该方法准确可靠 ,可用于双黄连胶囊制剂的质量控制
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| 关 键 词： | 双黄连胶囊 绿原酸 反相高效液相色谱法 |
| 文章编号： | 1000-1719(2004)08-0688-02 |
| 修稿时间： | 2004-05-21 |
Determination of chlorogenic acid in Shuanghuanglian Capsules by RP- HPLC |
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| CHEN Ji ying,ZHAO Hui,SHAN Zhen shun. Determination of chlorogenic acid in Shuanghuanglian Capsules by RP- HPLC[J]. Liaoning Journal of Traditional Chinese Medicine, 2004, 31(8): 688-689 |
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| Authors: | CHEN Ji ying ZHAO Hui SHAN Zhen shun |
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| Abstract: | Objective :To establish a HPLC method for the determination the content of chlorogenic acid in huanghusnglian Capsules. Method :Phenomenx- C 18 Column(250mm×4.6mm,5um)was used.The mobile phase was water-acetonitrile -glacial acetic acid (94∶5∶1.0).The detection wavelength was 326 nm and flow rate was 1 0ml·min -1 . Result :A linearity was obtained for chlorogenic acid ( r =0.9999),and the average recovery of chlorogenic acid was 99.11%,RSD was 0.58%( n =6). Conclusion :The method is reliable,accurate,and very suitable to the quality control of huanghusnglian Capsules. |
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| Keywords: | huanghusnglian Capsules Chlorogenic acid RP-HPLC |
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