乙型肝炎病毒消毒指标探讨

本文选用四种微生物际本,在进行物理与化学的消毒处理后,作反相间接血凝试验、DNA-P 试验及电镜检查,通过与常规培养结果的比较,阐明其与微生物活性之间的相关及其作为消毒效果评价指标的意义。实验结果表明,微生物抗原性破坏甚为困难,且与微生物本身的耐力不相关,而微生物体内聚合酶的存在与消失与微生物本身的存亡亦无同步相关,均不宜作为消毒效果的评价指标。在致死剂量的消毒处理后,电镜下可见细菌胞壁破坏,原浆外流,病毒颗粒减少与结构模糊,可望作为消毒效果评价的有效手段。

AN APPROACH TO SOME INDICATORS FOR DISINFECTION OF HBV

The samples of B coli,spores of B cereus,bacteriophage F2 and HBsAg positive sera,disinfected with moist heat,peracetic acid,Ca(OC1)_2 and chlorine taken from electrolysed NaC1 solution,were examined with culture,RPHA,DNA-polymerase test and electron microscope assay,in order to approach the meaning of these indicators in disinfection tests. The results of RPHA showed that it is too difficult to destroy the antigenicity of the tested microbes,and failed to confirm the relationship between the destruction of the antigenicity and tolerability of the same microbes.The activity of DNA-P or RNA-P,whether it is existed or not,did not synchronize with the activity of microbes itself.The authors therefore proposed that the destruction of the antigenicity and the inactivity of polymerase be not the radical factors of living microbes after disinfection. The features of electron microccope assay indicated that under the action of lethal doses of disinfectants,the rupture of cell membranes and effusion of plasma could be seen in the samples of B coli and the spores of B cereus.The decrease of viral granula number,and shrinkage and vagueness of the structure appeared in the samples of F2 and HBsAg positive sera.It is expected to establish a reliable indicator for disinfection of HBV by further improving the technique and promoting the resolving power of electron microscope.