p73、PTEN和Ki-67在子宫内膜样腺癌组织中的表达及意义

目的探讨p73、PTEN和Ki-67蛋白在子宫内膜癌前病变及内膜样腺癌中的表达及意义。方法用免疫组化SP法,在13例子宫内膜正常增生期、20例简单型增生过长、22例复杂型增生过长(包括11例不典型增生)及38例内膜样腺癌组织中检测p73、PTEN和Ki-67蛋白的表达情况。结果随着子宫内膜病变的恶性进展,p73和Ki-67蛋白的阳性表达率上调而PTEN下调,且p73和Ki-67表达上调呈显著正相关(P<0.01),Pearson列联系数为0.5144。p73和Ki-67蛋白表达与子宫内膜样腺癌组织分化程度有关(P<0.01),Ki-67和PTEN蛋白表达与子宫内膜样腺癌的临床分期和肌层浸润有关(P<0.05)。结论p73和Ki-67协同调控子宫内膜腺上皮细胞的生长、增殖,并促进癌变;PTEN表达下调是子宫内膜样腺癌的早发事件。联合检测p73、Ki-67和PTEN蛋白的表达可作为在子宫内膜癌早期诊断的参考指标并指导临床早期治疗。

Expression of p73, PTEN and Ki-67 Proteins in Endometrioid Adenocarcinoma and Their Significance

Objective To investigate significance and alterations in expression of p73, PTEN and Ki-67 proteins in endometrial carcinogenesis. Methods Immunohistochemistry was applied to detect expression of p73, PTEN and Ki-67 proteins in 13 normal proliferating endometria,20 simple hyperplasia,22 complex hyperplasia including 11 cases with atypical hyperplasia and 38 endometrioid adenocarcinomas. Results As malignant progression of lesions, the positive rates of p73 and ki-67 obviously increased ,but that of PTEN decreased. There was highly relationship between p73 and Ki-67 expression(P< 0.01 ),Pearson coefficient was 0.5144.Expression of p73 and Ki-67 was associated with the grade, and that of PTEN and Ki-67 was associated with clinical stage and myometrial invasion. Conclusion p73,together with Ki-67, regulates endometrial epithelial cell growth, proliferation and promotes cancer.The change of PTEN is a early event in endometrioid adenocarcinoma. The levels of p73 , Ki-67 and PTEN proteins are molecular markers for early diagnosis of endometrioid adenocarcinoma and can give direction to early clinical therapy.