拉伸应变和加载时间对人牙周膜成纤维细胞IL-1β分泌量的影响

研究拉伸应变和加载时间对体外培养人牙周膜成纤维细胞（PDLF）的白细胞介素-1β（IL-1β）分泌量的影响，将原代，传代培养成功的PDLF制备成实验用细胞，分为5组（n=3)，而后分别置于特制的加力装置上拉伸加载，加力至拉伸应变量分别为0%，8%，12%，16%和20%，加力时间为24，48，72h。用双抗ELISA法分析IL-1β的含量。结果表明，8%，12%和16%拉伸应变组IL-1β的分泌量随着加力时间和力值的增加而递增；在各个时间组，IL-1β分泌量在16%应变值外达到最高值；20%拉伸应变组，加力24h和48h,IL-1β分泌量明显高于0%应变组，与12%和16%应变组相比已开始下降，加力72h,IL-1β分泌量大幅度下降，已低于对应的0%应变组，在细胞生理应变承受范围内，静态机械拉伸应变能促进人牙周膜成纤维细胞IL-1β的分泌，但刺激效应随着加力时间的增加而减少。

The Effects of Tensile Strain and Loading Time on the Secretion of IL-1β of Human PDLF

This study aims at exploring the effects of tensile strain and loading time on the secretion of IL-1 beta of human periodontal ligament fibroblast. Five tensile strain values including 0%, 8%, 12%, 16%, 20% and three loading time including 24 h, 48 h, 72 h are set in this study. The prepared cell samples are mounted on the self-devised loading apparatus in vitro. The content of IL-1 beta in each sample was determined using double-antibody ELISA. The secretion amount of IL-1 beta per day is directly proportional to the loading time and tensile strain value in tensile strain group of 8%, 12%, 16%. The secretion amount of IL-1 beta reaches its maximum at tensile strain value of 16% in loading time groups. Loaded strain for 24 h and 48 h, the secretion amount of IL-1 beta at tensile strain value of 20% is obviously more than that at value of 0%, but the amount already begin to decrease apparently. Loaded strain for 72 h, the secretion amount of IL-1 beta decreases to a great extent that it is less than the amount at strain value of 0%. The tensile strain stimulates the secretion of IL-1 beta by human periodontal ligament fibroblast when the strain is under normal physiological extent, but the stimulation effect fades out as time goes on.