结核感染与发病状态下相关细胞因子、T细胞亚群的变化及临床应用研究

目的 :对结核菌感染免疫应答过程中相关细胞因子、T细胞亚群的变化情况进行观察研究 ,并使之应用于临床。方法 :分别采用ELISA、APAAP、单向琼脂免疫扩散等方法对相关免疫指标进行检测。结果 :结核菌感染者T细胞亚群CD3+T细胞、CD4 +细胞、sIL 2R水平、特异结核循环免疫复合物增高 ;肺结核患者CD4 +T细胞降低 ,IL 2活性下降 ,mIL 2R表达明显降低 ,TNF α水平降低 ,CD8+细胞升高 ,sIL 2R明显升高 ,IL 4、IL 10水平均增高 ,且进展期较好转期更为明显。结论 :IL 2、IL 4、IL 10、TNF α、sIL 2R和T细胞亚群在免疫应答过程中具有重要的作用 ,以上免疫指标可判断结核感染、发病和病情变化情况

Study on the clinical application of the changing of cytokines and T sub-cells in the infection and incidence of tuberculosis

Objective: To study the changing of the related cytokines and T sub-cells during the immune reaction of tuberculosis infection and to apply it to the clinical.Methods: Detecting the related immune indicators with ELISA APAAP and the one-way Agar immune diffusing methods respectively.Results: It increases that the T sub-cells.CD＋3-cell,CD4＋-cell,the level of SIL-2R,and the specific tuberculosis circular immune complex for tuberculosis infectious cases.But it decreases the CD4＋-cell,the activity of IL-2,the expressing of mIL-2R and the TNF-α level,while CD8＋-cell,SIL-2R and the IL-4,IL-10 level increase for tuberculosis cases and they increase more obviously when they are in progression than in improvement.Conclusion: It plays important roles in the immune reaction that IL-2,IL-4,IL-10,TNF-α,SIL-2R and T sub-cells.We can determine if they are in infection or incidence of tuberculosis and the changing of their situation according to the immune indicators mentioned above.