Sickle erythrocytes increase prostacyclin and endothelin-1 production by cultured human endothelial cells under flow conditions |
| |
Authors: | Shiu Yan-Ting McIntire Larry V Udden Mark M |
| |
Affiliation: | Cox Laboratory for Biomedical Engineering, Department of Chemical Engineering, Rice University, Houston, Texas, USA. |
| |
Abstract: | We investigated the effects of sickle erythrocytes on the production of vasotone mediators in endothelial cells (ECs) using an in vitro recirculating flow system. Sickle erythrocytes increased the EC production of two important vasoactivators, prostacyclin and endothelin-1, under venous wall shear stress conditions of 1dyncm2. The presence of interleukin-1 in the perfusion system, as a model for inflammatory cytokine effects, enhanced the overall amounts of released prostacyclin but did not affect the production of endothelin-1. This study demonstrates the effects of sickle erythrocytes on the function and metabolism of ECs under vascular flow environments. The altered production of vasoactivators may contribute to the vasotone instability and vasoocclusive crises in sickle cell anemia. |
| |
Keywords: | sickle cell anemia endothelial cells prostacyclin endothelin-1 shear stress |
本文献已被 PubMed 等数据库收录! |
|