镇静小鼠的非侵入式气道反应性测定法及药物作用

目的：建立并验证镇静小鼠气道反应笥的非侵入式测定法，探讨小鼠气道高反应性与气道炎症的关系。方法：观察致敏及药物对小鼠引喘阈浓度的影响，及支气管－肺泡灌洗液（ＢＡＬＦ）中白细胞渗出量的变化。结果：与未致敏小鼠相比，致敏小鼠吸入ＯＡ６ｈ的ＭＣｈ引喘阈逍度显著降低，ＢＡＬＦ中细胞渗出量显著增高，地塞米松（７．５ｍｇ／ｋｇ）和氨茶碱（３７．５ｍｇ／ｋｇ）可降低致敏小鼠吸入ＯＡ引起的气道反应性增高和ＢＡＬＦ

A new non invasive method for the measurement of airway responsiveness in sedated mice and effects of drugs

OBJECTIVE:To establish and verify a non invasive method for quantitative measurement of airway responsiveness(AR) in diazepam sedated mice,and to investigate the relationship between airway hyperresponsiveness and airway inflammation in mice.METHOD:The effects of sensitization and drugs on the wheezing threshold concentration to methacholine(MCh),and the changes of the number of leukocytes in bronchoalveolar lavage fluid(BALF) in mice were observed.RESULTS:Lower wheezing threshold concentration to methacholine and more leukocytes in BALF were found in ovalbumin(OA) sensitized mice than those in non sensitized mice repectively after repeated antigen inhalation(0.5%,20 min per day,for 6 days).Dexamethasone(7.5mg/kg,ip.bid,for 3 days) and aminophylline(37.5mg/kg,ip.bid,for 3 days) inhibited these effects induced by antigen.CONCLUSION:This mouse method is useful to detect the changes in AR of mice,and there was a relationship between the airway hyperresponsiveness and airway inflammation induced by antigen in the sensitized mice.