| 桥式生物素—亲合素标记提高藻胆蛋白免疫荧光法灵敏度的研究 |
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| 引用本文： | 吴萍,顾铭,戚艺华,欧阳藩. 桥式生物素—亲合素标记提高藻胆蛋白免疫荧光法灵敏度的研究[J]. 细胞与分子免疫学杂志, 2001, 17(4): 321-323 |
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| 作者姓名： | 吴萍 顾铭 戚艺华 欧阳藩 |
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| 作者单位： | [1]中国科学院化工冶金研究所生化工程国家重点实验室，北京100080 [2]中国科学院化工冶金研究所生化工程国家重点实 |
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| 基金项目： | 国家 863计划海洋生物技术课题资助，No.819-07-04 |
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| 摘 要： | 目的 提高藻胆蛋白免疫荧光法检测的灵敏度。方法 在藻胆蛋白免疫荧光法（PBPIFA）的检测流程中，引入桥式亲合素-生物素系统（BRAB），称为BRAB-PBPIFA，用ELISA法与藻胆蛋白免疫荧光直接法和桥式BAS法，对定值的标准质控抗原HBsAg样品及500份血清样品同时进行测定，结果 BRAB应用于藻胆蛋白免疫荧光法中，能显著提高该法对低抗原含量样品的检出，对弱阳性血清的检出率可提高1.5倍，无蛋白封闭试剂Tween20在BRAB-PBPIFA中具有较好的封闭效果，结论 BRAB标记可提高藻胆蛋白免疫荧光法检测的灵敏度。
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| 关 键 词： | 桥式亲合素－生物素标记技术 荧光标记 藻胆蛋白免疫荧光 灵敏度 |
| 文章编号： | 1007-8738(2001)04-321-03 |
| 修稿时间： | 2000-12-12 |
Study on bridged avidinbiotin technique improving the sensitivity of phycobiliprotein immunofluorescence assay |
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| WU Ping,GU Ming,QI Yi-hua,OU-Yang Fan. Study on bridged avidinbiotin technique improving the sensitivity of phycobiliprotein immunofluorescence assay[J]. Chinese journal of cellular and molecular immunology, 2001, 17(4): 321-323 |
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| Authors: | WU Ping GU Ming QI Yi-hua OU-Yang Fan |
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| Affiliation: | State Key Laboratory of Biochemical Engineering Institute of Chemical MetallurgyChinese Academy of Sciences |
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| Abstract: | Aim To improve the sensitivity of phycobiliprotein immunofluorescence assay(PBPIFA), by introducing into bridged avidin biotin(BRAB) technique,known as BRAB-PBPIFA. Methods Using ELISA as a gold standard, effectiveness of three assays(direct PBPIFA, BRAB-PBPIFA and ELISA)were compared in simultaneous detection of standard antigen and 500 serum samples. Results BRAB improved greatly sensitivity of PBPIFA in detecting antigen of low concentration, and detection rate of weakly positive samples could increase by one times and a half. In the applying, protein-free blocking agent Tween 20 to BRAB-PBPIFA,possessed better blocking effect. Conclusion Combination of BRAB technique with PBPIFA can improve the detcctive sensitivity. |
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| Keywords: | bridged avidin biotin labeling technique fluorescence labeling phycobiliprotein immunoflluorescence assay |
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