胃癌组织中人巨细胞病毒感染及其临床意义

目的：初步探讨人巨细胞病毒（HCMV）感染与胃癌的关系。方法：采用化学发光（CLIA）法对胃癌组（n=80）及健康对照组（n=80）的外周血清进行HCMV Ig G、Ig M抗体检测;巢式PCR技术检测22例胃癌患者的肿瘤及癌旁正常胃黏膜组织标本中的HCMV UL55基因;统计分析HCMV感染与胃癌临床特征的关系。结果：胃癌组血清HCMV-Ig G阳性率为95%（76/80）,HCMV-Ig M阳性率为6.25%（5/80）;在健康对照组血清中,HCMV-Ig G阳性率为97.5%（78/80）,HCMV-Ig M阳性率为3.75%（3/80）,HCMV Ig G、Ig M在胃癌组与健康对照组血清中差异无统计学意义（P〉0.05）。HCMV UL55阳性检出率在22例胃癌组织中为50%（11/22）,在相应正常胃黏膜组织中为9.09%（2/22）,胃癌组织HCMV阳性检出率较癌旁正常组织显著升高（P〈0.01）。此外,未发现HCMV感染与胃癌的TMN分期、组织病理分化及肿块大小有显著性关联。结论：胃癌组织中存在HCMV感染,且HCMV相较于癌旁正常组织更倾向感染癌灶,提示HCMV感染可能参与胃癌的发生发展过程。

Primary research of the relationship between HCMV infection and gastric cancer

Objective： To explore the correlation between HCMV infection and gastric cancer. Methods：The specific IgG and IgM antibodies of HCMV in serum of gastric cancer group （n=80） and health control group （n=80） were detected using the chemiluminesence immunoassay. HCMV infection in gastric cancer tissues and corresponding normal tissues were determined through the detection of UL55 gene by Nested PCR. The rela- tionships between HCMV infection and clinical features of gastric cancer were further analyzed. Results： The positive rate of HCMV-IgG and IgM were 95% （76/80） and 6.25% （5/80） in gastric cancer group, respectively. Meanwhile, the positive rate were 97.5% （78/80） and 3.75% （3/80） in health control. There was no statistically significant difference between the gastric cancer and health control group in HCMV-IgG, IgM detection. How- ever, there was a significant difference between gastric cancer tissues and corresponding normal tissues in HCMV detection. The positive rate of HCMV UL55 was 50% （11/22） in gastric cancer tissue samples, whereas 9.09% （2/22） in corresponding normal tissues through Nested PCR detection. In addition, There was no obvious associa- tion between HCMV infection and TMN stage, pathological differentiation type and the size of tumor. Conclu- sion： It is the first time to report the HCMV infection in gastric cancer tissues, and the HCMV may preferentially infect the neoplastic epithelium than corresponding normal epithelium, this research suggests the HCMV infec- tion may participate in the occurrence and development stages of gastric cancer.