银杏内酯B、C及联合用药预处理对大鼠心肌缺血/再灌注心功能的影响

目的 探讨银杏内酯B（ginkgolide B,GB）、银杏内酯C（ginkgolide C,GC）及两者1∶1联合用药预处理对心肌缺血/再灌注损伤（ischemia/reperfusion injury,I/R）大鼠血流动力学的影响及其与氧自由基的关系.方法 50 只SD大鼠,随机分为5 组（n=10）：正常对照组（Control组）、缺血/再灌注组（I/R组）、银杏内酯B预处理组（GB组）、银杏内酯C预处理组（GC组）及银杏内酯B和C联合用药预处理组（GB＋GC组）.采用Langendorff装置逆行插管灌流,建立大鼠离体心脏I /R 模型;除Control组外,其余各组均缺血20 min,再灌注40 min.记录左心室收缩压（LVSP）、左心室舒张末压（LVEDP）、左心室内压上升和下降的最大变化速率（＋dp/dtmax）,并测定冠状动脉流出液中乳酸脱氢酶（LDH）的活性.结果 GB组、GC组及GB＋GC组用药预处理后,复灌时左心室内压差（△LVP）、＋ dp/dtmax等心功能指标明显优于I/R组（P〈0.05）,联合用药较单一用药效果差异不明显,各用药组的LDH活性均较I/R组明显降低.结论 银杏内酯B、C及联合用药均能一定程度改善大鼠心肌缺血/再灌注损伤,但联合用药较单一用药效果优势不明显.

Effects of ginkgolide B,ginkgolide C and their combined therapy on the cardiac function of rats with ischemia/reperfusion injury

Objective To explore the effects of ginkgolide B, ginkgolide C and their combined therapy on the cardi- ac function of rats with ischemia/reperfusion injury. Methods Fifty Sprague - Dawley rats were randomly divided into five groups： a normal control group （Control group）, an ischemia/reperfusion injury group （I/R group）, a ginkgolide B pretreatment group （GB group）, a ginkgolide C pretreatment group （GC group） and a combined pretreatment group （GB ＋ GC group）. Langendorff apparatus was adopted to perform retrograde perfusion, and an in vitro isolated rat heart model of I/R injury was established. Each group except the control underwent ischemia operation for 20 minutes followed by reperfusion for 40 minutes. Then, we recorded left ventricular systolic pressure （ LVSP）, left ventricular end diastolic pressure （ LVEDP）, the maximal rate of systolic pressure rise （ ＋ dp/dtmax ） and the maximal rate of diastolic＇ pressure decrease （ - dp/dtm,x ） and detected the activity of LDH in the fluid of coronary artery. Results During reperfusion, the GB group, GC group and GB ＋ GC group presented remarkable increases in LVP and decreases in the activity of LDH （ P 〈 0.05 ）, There was no significant change between the effects of lhe （;B ＋ GC group and GB or GC group, Conclusion GB, GC and their combination can improve the cardiac fu.ction of rats with I/R injury, but the combined pretreatment did not present advantages over GB or GC alone.