| 双阻滞位点特异性PCR鉴别铁皮石斛及其近缘物种 |
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| 引用本文： | 董晓曼,蒋超,袁媛,查良平,彭代银,赵玉洋.双阻滞位点特异性PCR鉴别铁皮石斛及其近缘物种[J].中国中药杂志,2017,42(5):896-901. |
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| 作者姓名： | 董晓曼 蒋超 袁媛 查良平 彭代银 赵玉洋 |
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| 作者单位： | 安徽中医药大学 药学院, 安徽 合肥 230012;中国中医科学院 中药资源中心 道地药材国家重点实验室培育基地, 北京 100700,中国中医科学院 中药资源中心 道地药材国家重点实验室培育基地, 北京 100700,中国中医科学院 中药资源中心 道地药材国家重点实验室培育基地, 北京 100700,安徽中医药大学 药学院, 安徽 合肥 230012,安徽中医药大学 药学院, 安徽 合肥 230012;安徽中医药大学 药学院 现代中药安徽省重点实验室, 安徽 合肥 230012,中国中医科学院 中药资源中心 道地药材国家重点实验室培育基地, 北京 100700 |
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| 基金项目： | 中医药行业科研专项（201407003） |
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| 摘 要： | 建立一种快速、准确鉴别铁皮石斛加工制品的方法。该研究通过对Gen Bank数据库收录的铁皮石斛及其同属近缘物种的ITS序列进行对比分析,根据变异位点设计双阻滞位点特异性鉴别引物,并优化PCR条件,对铁皮石斛及其69种近缘种共362个样品进行扩增和检测。结果显示,在退火温度为60℃的同一PCR反应中,铁皮石斛均扩增出397 bp条带,而其他69种近缘物种均无条带,且该方法灵敏度高,检出限可达0.03 mg·L~(-1)。该文所建立的位点特异性PCR方法可实现铁皮石斛的准确鉴别,具有操作简便、稳定可靠、应用范围广的优点。
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| 关 键 词： | 铁皮石斛 位点特异性PCR 分子鉴定 |
| 收稿时间： | 2016/9/28 0:00:00 |
Study on identification of Dendrobium officinale and related species by bidirectional PCR amplification of mismatched and specific alleles |
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| DONG Xiao-man,JIANG Chao,YUAN Yuan,ZHA Liang-ping,PENG Dai-yin and ZHAO Yu-yang.Study on identification of Dendrobium officinale and related species by bidirectional PCR amplification of mismatched and specific alleles[J].China Journal of Chinese Materia Medica,2017,42(5):896-901. |
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| Authors: | DONG Xiao-man JIANG Chao YUAN Yuan ZHA Liang-ping PENG Dai-yin and ZHAO Yu-yang |
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| Institution: | School of Pharmacy, Anhui University of Chinese Medicine, Hefei 230012, China;State Key Laboratory of Dao-di Herbs Breeding Base, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China,State Key Laboratory of Dao-di Herbs Breeding Base, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China,State Key Laboratory of Dao-di Herbs Breeding Base, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China,School of Pharmacy, Anhui University of Chinese Medicine, Hefei 230012, China,School of Pharmacy, Anhui University of Chinese Medicine, Hefei 230012, China;Key Laboratory of Modern Traditional Chinese Medicines of Anhui Province, School of Pharmacy, Anhui University of Chinese Medicine, Hefei 230012, China and State Key Laboratory of Dao-di Herbs Breeding Base, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China |
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| Abstract: | |
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| Keywords: | Dendrobium officinale PCR amplificationof specificalleles molecularidentification |
| 本文献已被 CNKI 等数据库收录！ |
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