间质胶原酶表达质粒对实验性大鼠肝纤维化的影响

目的观察间质胶原酶（ＭＭＰ－１）表达质粒对大鼠肝纤维化的影响。方法运用逆转录巢式ＰＣＲ和重组ＤＮＡ技术，构建大鼠ＭＭＰ－１真核细胞表达质粒，经脂质体包埋后，利用腹腔注射将其导人免疫性大鼠肝纤维化模型体内；通过ＲＴ－ＰＣＲ，Ⅰ、Ⅲ型胶原的免疫组化以及Ｍａｓｓｏｎ胶原染色观察ＭＭＰ－１表达质粒对大鼠肝纤维化的影响。结累ＭＭＰ－１表达质粒可促进肝脏中Ⅰ、Ⅲ型胶原的降解（Ｐ＜０．０１），且作用较秋水仙碱为强（Ｐ＜０．０５）；在病理形态学的观察方面，ＭＭＰ－１表达质粒对肝纤维化有一定的逆转作用（Ｐ＜０．０５），但与秋水仙碱相比差异无显著性（Ｐ＞０．０５）。结论ＭＭＰ－１表达质粒对肝纤维化有一定的逆转作用，但作用有限。

Effects of MMP-1 expressing plasmid on rat liver fibrsis

Objective To observe tile effects of MMP-1(Matrix Metalloproteinase-1) expressing plasmid on rat liver fibrosis. Methods We constructed the rails MMP-1 recombinant plasmid which can beexpressed in eucaryotic cells. After encapsLilating the MMP-1 recombinant plasmid by lipofectin, we transferred it to the rails fibrotic liver in vivo intraperitoneally, then observed the effects of the plasmid onthe fibrotic liver by RT-PCR, immunohistochemistry and the observation of pathology. Results Therecombinant plasmid of MMP-1 could increase the degradation of type 1 and type ill collagen in therails fibrotic liver according to immunohistochemistry compared with fibrotic modal gToup(P<0.0l) or compared with colchicine group(P<0.05). According to the observation of pathology, recombinant plasmid ofMMP-l has some reverse eclats on the rat's fibrotic liver compared with fibrotic model group(P<0.05),but noobvious difference was found between the plasmid and colchicine(P>0.05). Conclusion The recombinantplasmid of MMP-1 has some reverse effects on liver fibrosis, but the effects is limited.