体外生物人工肝支持作用的实验研究

目的 构建理想的体外人工肝支持系统，探讨其对暴发性肝衰竭的支持作用。方法 用经体外两步灌流法分离，限制贴壁技术球形聚集混合培养的人肝细胞－肝非实质细胞与中空纤维型生物反应器及由血液透析仪改装的辅助循环装置共同构建的ＥＢＬＳＳ，对门腔静脉分流加入肝血液阻断法建立的ＦＨＦ模型犬进行人工肝支持实验。结果 在平均约１０＾８肝细胞－肝非实质细胞ＥＢＬＳＳ的支持下，治疗组犬存活时间较无细胞空白循环对照组延长近

Liver support effect of experimental extracorporeal bioartificial liver

OBJECTIVE: To establish an extracorporeal bioartificial liver support system (EBLSS) and study its support effect for fulminant hepatic failure (FHF). METHODS: The EBLSS consists of three parts: human liver cells (hepatocytes, nonparenchymal hepatocytes) isolated and cultured by extracorporeal two-stage perfusion and spheroidal aggregates methods respectively, hollow fiber bioreactor, and supplementary circulation unit, which was employed to support the FHF model dogs. RESULTS: Compared with the control dogs treated with EBLSS but without liver cells, the survival time of artificial liver support dogs was significantly prolonged, the changes of blood pressure, heart rate and ECG were slower, and the serum ammonia and lactate levels were significantly lower at the 3 and 5 hr. In addition, a good viability of human liver cells was noted after 5 hr experiment. CONCLUSION: This EBLSS has played the metabolic role of cultured heman hepatocytes, is capable of compensating the function of the liver, and might provide effective artificial liver support and therapy for patients with FHF or severe hepatitis.