HepG2.2.15细胞诱导MT2淋巴细胞凋亡及其意义

观测表达Ｆａｓ抗原（Ｆａｓ）的Ｔ淋巴细胞一感染乙型肝炎病毒的ＭＴｓ细胞对表达Ｆａｓ配体（ＦａｓＬ）的实质细胞一ＨｅｐＧ２２．１５细胞的敏感性，了解乙型肝炎病毒感染对淋巴细胞和肝实质细胞交互作用的影响。方法体外培养ＭＴ２细胞，用乙型肝炎病毒诱导表达ＦａｓＬ。将表达ＦａｓＬ的ＨｅｐＧ２．２．１５细胞与其共孵育后，以Ｔｕｎｅｌ法观测ＭＴ２细胞凋亡情况。结果ＭＴ２细胞感染乙型肝炎病毒后可测到Ｆａｓ表达信号。与表达ＦａｓＬ的ＨｅＰＧ２．２．１５细胞共孵育４８～７２小时以后，出现凋亡信号。结论乙型肝炎病毒诱导表达Ｆａｓ的ＭＴｓ细胞与表达ＦａｓＬ的ＨｅｐＧ２．２．１５细胞交互作用后发生凋亡，表明通常在乙型肝炎发病中作为效应细胞的淋巴细胞，亦可成为凋亡靶细胞。

Apoptosis in MT2 cells induced by HepG2.2.15 tells

Objective To obtain the apoptosis in MT2 cells induced by HepG2.2.15 cells expressingFas Ligand. Methods A human T-lymphotropic virus type I infected cell line Ma2 was culturedovernight, then 0.5ml serum contained hepatitis B virus DNA(HBV DNA), E antigen and S antigen wasadded to it together with I ml of fresh RPMI 1640 medium and incubated for 20~ 24 hours at 37. Thecells were washed three times with RPMI 1640 medium without serum, followed by continued incubation.The cells were harvested and Fas antigen were determined by immunohistochemistry. The cells expressedFas antigen was added to HepG2.2.15 cells expressing Fas Ligand together with fresh RPMI 1640 mediumand incubated for 48 ~ 72 hours at 37. The apoptosis in MT2 cells was detected by terminaldeoxynucleotidyl transferase mediated dUTP nick end labeling (TUNEL). Results The Fas antigen wasobserved in MT2 cells harvested at 8 ~ 12 days post-infected, and the strong signals of apoptosis wasobserved in MT2 cells incubated together with HepG2.2.15 cells for 48~ 72 hours. Conclusion MT2, ahuman T cell line infected with hepatitis B virus is able to express Fas antigen. The apoptosis in MDcells induced by HepG2.2.15 indicated that the T-lymphocyte in the quality of effective cell and thehepatocyte in the quality of target cell could be reversed in the pathogrnesis of viral hepatitis.