三螺旋形成寡核苷酸抑制乙型肝炎病毒复制及抗原合成的实验研究

目的 探讨三螺旋形成寡核苷酸抗乙型肝炎病毒（ＨＢＶ）作用。方法 针对ＨＢＶ核心启动子ＳＰ１位点，合成２１ｍｅｒ硫代磷酸三螺旋形成寡核苷酸及２１ｍｅｒ无关对照寡核苷酸。采用ＬＥＩＳＡ，斑点杂交法分别检测了经寡核苷酸处理的ＨｅｐＧ２．２．１５细胞及空白对照组细胞培养上清ＨＢｓＡｇ，ＨＢｅＡｇ及ＨＢＶ ＤＮＡ水平。结果 ＴＦＯ２１组２．２．１５细胞ＨＢｓＡｇ及ＨＢＶ ＤＮＡ分泌量明显低于空白对照组。ＴＦ

Inhibitory effect of triplex forming oligodeoxynucleotides on HBV replication and synthesis of antigen

Objective To explore the inhibitory effect of triplex forming oligodeoxynucleotides (T F O )on replication of HBV and synthesis of antigen. Methods A 21 mer phosphorothioate triplex formingoligodeoxynucleotides (TFO21) directed at SPl sites in HBV core promoter and a control of 21 inerphosphorothioate oligodeoxynucleotides (ODNcon) were synthesized. HepG 2.2.15 cells which can produceHBsAg, HBeAg, HBV DNA and HBV particle were treated with TFO21 and ODNcon. In HepG 2.2.15 cellstreated with these oligodeoxynucleotides, the levels of HBsAg, HBeAg, and HBV DNA were examined byELISA and dot hybridization method, respeCtively. Results The levels of HBsAg, HBeAg and HBV DNAin TFO21 gToup were lower than those in the bank control group. At concentration of 10 mol/L, TFO21inhibited snythesis of HBsAg and HBeAg by 57.5% and 77%. The inhibitory effect of TFO21 was dosagrdependent, and was related to time in which 2.2.15 cells were incubated with TFO21. No inhibition wasobserved in the ODNcon group. No toxicity was observed in the 2.2.15 cells treated witholigodeoxynucleotides. Conclusion These results indicate that TFO is a potent inhibitor for HBVreplication and synthesis of antigen, and also suggest that TFO is a therapeutic potential for the treatmentof patients infected with HBV.