非甲－非庚型肝炎肝组织中经输血传播病毒DNA的原位检测

目的探讨非甲－非度型病毒性肝炎患者肝组织中经输血传播病毒（ｔｒａｎｓｆｕｓｉｏｎｔｒａｎｓｍｉｔｔｅｄｖｉｒｕｓ，ＴＴＶ）ＤＮＡ的表达。方法采用地高辛素标记ＴＴＶＤＮＡ探针以原位杂交技术对１９例血清学甲、戊型肝炎病毒标志物、免疫组织化学检测肝组织中ＨＧＶＮＳＳ均用性的急、慢性病毒性肝炎患者肝组织进行检测。结果１９例肝组织中６例检出ＴＴＶ基因阳性（３１．５８％），其中急性轻型肝炎４例（３０．８０％），慢性肝炎２例（３３．３３％）。ＴＴＶＤＮＡ表达于肝细胞核或胞浆内。在急性肝炎，ＴＴＶ阳性细胞弥漫分布于肝小叶内，慢性肝炎于汇管区附近较为密集。６例中５例患者有转氨酶增高。结论非甲－非庚型病毒性肝炎肝组织中ＴＴＶＤＮＡ的检出表明ＴＴＶ为一种新型的嗜肝性病毒。

Detection of TTV DNA in liver tissue of patients with non A-G hepatitis

Objective To study the existence of TTV (transfusion-transmitted virus) in liver tissueof patients with non A-G hepatitis. Methods 19 cases of paraffin-emboded liver tissues of the patientswith non A-G tiepatitis were studied by in sity hybridhation with Die-TTV DNA probe. Results Outof 19 caac, TTV DNA were detected in 6 cases, including 4 cases of acme mild hepatitis (30.80%) and 2cases of chronic hepatitis(33.33%), respectively. Hybridization shoals were daftted within the nuclei orcytoplasm of the hepatocytes. The positive cells were scattered in the intralobular areas in acute mildhepatitis and more aggregated in peripohal areas in chronic hepatitis. Among the 6 positive cases, 5 hada raised ALT. Conclusion The result showed that the TTV detected in liver tissue of patients withunknown etiology is a novel virus, watch belongS to an hepadnavirus.