| 几种登革热抗体检测试剂的比较 |
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| 引用本文： | 冯翔宇,张华荣,刘启军,曾令聪,雷红宇,陈正明. 几种登革热抗体检测试剂的比较[J]. 旅行医学科学, 2009, 15(3) |
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| 作者姓名： | 冯翔宇 张华荣 刘启军 曾令聪 雷红宇 陈正明 |
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| 作者单位： | 1. 重庆出入境检验检疫局,重庆,400020
2. 重庆国际旅行卫生保健中心,重庆,401147 |
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| 摘 要： | 目的比较2种登革热抗体ELISA检测试剂盒以及1种ICT(免疫层析法)试剂盒对于登革热抗体检测的差异,筛选出更适合群体血清流行病学检测的试剂盒。方法按照所选择的试剂盒的说明,用盲法对出入境人员血清进行检测。结合检测和流调结果进行分析。结果登革热IgG阳性检出率分别是NOV16.67%、中山10.12%、Cotez0.6%,总体上检出率差异有统计学意义(P<0.05),中山和NOV之间检出率差异没有统计学意义(P>0.05);登革热IgM阳性检出率分别是NOV0%、中山1.19%、Cotez3.57%,三者检出率差异均有统计学意义(P<0.05)。分析三种试剂检测阳性与黄热、乙脑接种、病史的关系,提示Cortez-IgG呈现弱相关趋势。检测成本测算,中山试剂成本最低。结论对于开展人群登革热血清流行病学调查和监测,中山-IgG试剂相对较优。不同检测原理、不同工艺的试剂盒,其检测特性需要进一步研究和评估。
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| 关 键 词： | 登革热 抗体 检测试剂 |
Comparison of Several Reagents for Detecting DF Antibody |
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| Affiliation: | FENG Xiang-yu1ZHANG Hua-rong 2LIU Qi-jun 1et al (1.Chongqing Entry-exit Inspection and Quarantine Bureau; Chongqing 400020; 2.Chongqing International Traveler Health Care Center; Chongqing 401147); |
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| Abstract: | Objective To find out a suitable reagent for dengue seroepidemiology,two ELISA reagents(Novatec and zhongshan)and ICT reagent(Cortez)were applied to detect dengue antibody.Methods According to reagent manual,blood samples collected randomly from entry-exit population were detected by 3 kinds of reagent.Then data from detecting were analyzed.Results The IgG positive rate were 16.67%(NOV),10.12%(zhongshan),0.6%(Cortez)respectively.There was statistical difference between 3 kinds of reagent(P<0.05).But ther... |
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| Keywords: | Dengue Antibody Detection reagent |
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