| 当归内酯类成分的结构鉴定与抗氧化活性筛选 |
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| 引用本文： | 李伟东,吴育,刘雪东,蔡宝昌.当归内酯类成分的结构鉴定与抗氧化活性筛选[J].中成药,2011,33(12):2114-2118. |
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| 作者姓名： | 李伟东 吴育 刘雪东 蔡宝昌 |
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| 作者单位： | 南京中医药大学江苏省中药炮制重点实验室; |
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| 基金项目： | 江苏省高校优势学科建设工程资助项目(ysxk-2010); 江苏省高校自然科学基金研究项目(07KJD360163) |
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| 摘 要： | 目的分离当归中内酯类成分,并对其血管内皮细胞保护活性进行筛选。方法当归经CO2超临界萃取,萃取物通过硅胶柱层析分离得到单体成分,波谱法鉴定其结构;采用过氧化氢(H2O2)建立体外培养的人脐静脉内皮细胞(HU-VEC)氧化应激损伤模型,设立模型组、对照组和药物组,其中药物组给予5种当归内酯类成分预培养24 h后加入25mmol/LH2O2,四甲基偶氮唑盐(MTT)法检测细胞存活率。结果分离得到(Z)-藁本内酯(Ⅰ),(Z)-正丁烯基苯酞(Ⅱ),exo-Z,Z’-3α.7α’,7α.3α’-双藁本内酯(Ⅲ),3,9-二羟基藁本内酯(Ⅳ),(Z,Z’)-6.8’,7.3’-双藁本内酯(Ⅴ)等5种内酯类成分。其中(Z)-藁本内酯、3,9-二羟基藁本内酯具有显著的血管内皮细胞保护活性。结论当归中某些内酯类成分具有较强的抗氧化能力及内皮细胞保护作用,符合当归的功效、主治。
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| 关 键 词： | 当归 内酯类成分 人脐静脉内皮细胞 活性筛选 |
Isolation,identification and screen of lactone compounds from Angelica sinensis |
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| LI Wei-dong,WU Yu,LIU Xue-dong,CAI Bao-chang.Isolation,identification and screen of lactone compounds from Angelica sinensis[J].Chinese Traditional Patent Medicine,2011,33(12):2114-2118. |
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| Authors: | LI Wei-dong WU Yu LIU Xue-dong CAI Bao-chang |
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| Institution: | LI Wei-dong,WU Yu,LIU Xue-dong,CAI Bao-chang*(Jiangsu Provincial Key Laboratory of Chinese Medicine Processing,Nanjing University of Chinese Medicine,Nanjing 210029,China) |
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| Abstract: | AIM To isolate and screen out some angelica lactone components from Angelica sinensis which protect vascular endothelial cells.METHODS CO2 supercritical fluid extraction was used to obtain crude extract of Angelica sinensis,and then extract ran through silica gel column chromatography to get angelica lactone monomer and their structures were identified by spectral analysis.Using hydrogen peroxide-induced cultured human umbilical vein endothelial cells(HUVEC) oxidative stress model in vitro was divided into ... |
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| Keywords: | Angelica sinensis lactone compounds HUVEC activity screening |
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