参麦注射液对DPPH自由基所致兔离体心脏功能损伤的保护作用

目的 :在兔离体灌流心脏观察参麦注射液对DPPH自由基所致心功能损伤的保护作用。方法 :离体兔心采用Langendorff法灌流 ,记录左室内压 (LVP)、左室内压上升速率 (+dp/dtmax)、左室舒张末压 (LVEDP)、心率(HR) ,定时收集冠脉流出液测量冠脉流量 (CF)和肌酸激酶 (CK)活性。结果 :用含 0 .2 5 μmol/LDPPH的K H液灌流心脏 1 0min ,LVP ,+dp/dtmax明显降低 ,LVEDP升高 ,冠脉流量中的CK值明显增加。用含参麦注射液(1 :32 0 )的K H液灌流心脏 1 0min ,可显著减轻DPPH所致的心功能损伤 ,减少CK的释放。结论 :参麦注射液对DPPH自由基所致心功能损伤具有明显保护作用

Protective effects of shenmai injection on DPPH free radical-induced injuries in isolated hearts in rabbits

Objective To examine the protective effects of shenmai injection (SM) on DPPH free radical induced myocardial injury in isoiated hearts in rabbits. Methods The isolated rabbit hearts were perfused in a Langendorff model. The left ventricular pressure (LVP), the first derivative of LVP (+dp/dtmax),left ventricular end diastolic pressure (LVEDP) and heart rate(HR) were recorded. Coronary flow (CF) and creatine Kinase (CK) of coronary issue were measured. Results Perfusion with DPPH (0.25 μmol/L) for 5 min caused a significant impairment of cardiac function, as shown by a decrease in LVP and +dp/dtmax and an increase in LVEDP as well as the increased release of CK. Pretreatment with shenmai injection (1:320) for 10 min significantly improved the cardiac function and decreased the release of CK. Conclusion Shenmai injection protects against the myocardial injury induced by free radical (DPPH) in isolated rabbit hearts.