白芷香豆素的镇痛作用部位及其机制

目的 观察白芷香豆素(Coumarin of Angelicae Dahuricae,CAD)的镇痛作用部位及其机制.方法 利用小鼠福尔马林实验及侧脑室注射CAD对小鼠热板痛反应潜伏期的影响以分析CAD的镇痛作用部位;观察CAD对甲醛所致伤害性疼痛模型小鼠血清一氧化氮(NO)和脑组织中β-内啡肽(β-EP)含量的影响.结果 CAD(30,60,120 mg/kg)不同程度地抑制了小鼠福尔马林实验第一和第二时相反应.侧脑室注射CAD(6 mg/kg)明显延长小鼠热板痛反应潜伏期.CAD(30,60,120 mg/kg)连续给药4 d,使甲醛所致伤害性疼痛模型小鼠血清NO含量和脑中β-EP含量明显下降.结论 CAD对物理、化学等伤害性刺激具有明显镇痛作用,作用部位可能既在中枢也在外周,镇痛机制可能与抑制NO的合成或释放有关.

Studies on analgesic site and mechanisms underlying of Coumarins of Angdicae Dahuricae

Objective To observe analgesic site and mechanisms underlying of Coumarins of Angdicae Dahuricae (CAD).Methods The anti-nociceptive site of CAD was analysed with mice formalin test and mice hotplate test after intracerebroventricular (icv) administration;the concentration of nitric oxide (NO) in serum and β-EP in brain of mice were measured with nitric reductase method and radioimmunoassay (RIA) respectively.Results CAD (30,60,120 mg/kg) reduced the first- and second-phase responses of mice formalin test compared with control group.After one time icv administration of CAD(6 mg/kg) for mice,hotplate latency were significantly prolonged.The concentration of NO in serum and β-EP in brain of mice induced by formalin were both decreased.Conclusions CAD could induce definite analgesic effect,which may be related to central and peripheral mechanisms.