脐带间质干细胞移植治疗对MRL/lpr狼疮鼠单核细胞趋化蛋白-1的影响

目的:探讨脐带间质干细胞(UC-MSCs)治疗MRL/lpr狼疮鼠的作用机制。方法:24只MRL/lpr鼠随机分为UC-MSCs1次移植治疗组(G1)、UC-MSCs3次移植治疗组(G2)、对照组(G3)。酶联免疫吸附法检测血清和尿液单核细胞趋化蛋白-1(MCP-1)水平,蛋白印迹法检测肾脏MCP-1蛋白水平的表达,实时定量PCR检测肾脏MCP-1基因mRNA表达,免疫组化检测MCP-1蛋白在肾脏表达。结果:(1)29周时G2组血液MCP-1水平为827.70±259.36pg/mL,显著低于G3组1 325.45±352.28pg/mL(P<0.05);28周时G2组尿液MCP-1为239.93±31.47pg/mL,显著低于对照483.52±184.37 pg/mL(P<0.01)。(2)G2组(0.30±0.02)和G1组(0.40±0.02)MCP-1蛋白表达与G3(0.67±0.05)组比较差异有统计学意义(P<0.01);G2组也显著低于G1组(P<0.05)。(3)G2组(0.30±0.01)和G1组(0.39±0.02)MCP-1基因mRNA表达显著低于对照组(0.79±0.15)(P<0.05),G2组显著低于G1组(P<0.05)。(4)MRL/lpr鼠MCP-1主要定位于肾小球系膜区和肾间质炎性细胞浸润处及肾小管。G3组肾小球系膜区强表达MCP-1,治疗G1、G2组表达明显减弱。结论:UC-MSCs可能通过抑制MCP-1表达而发挥治疗作用。

The effect of umbilical cord mesenchymal stem cells transplantation for the MRL/ lpr mice on chemokine monocyte chemoattractant protein-1

Objective：To investigate the therapeutic mechanism of umbilical cord mesenchymal stem cell（UC-MSCs）transplantion for the MRL/lpr mice.Methods：Twenty four 18-week-old MRL/lpr female mice were divided into 3 groups as following： Group 1（G1）have been transplanted with1×106 UC-MSCs through caudal vein,Group 2（G2）have been transplanted with1×106 UC-MSCs three times and Group 3（G3）have been treated with 0.5ml Sodium Chloride as the control.Enzyme linked immunosorbent assay（ELISA） was used to measure the level of monocyte chemoattractant protein-1（MCP-1） in urine and blood serum and the expression of MCP-1 protein was mearsured by western blot.The expression of MCP-1 gene mRNA was detected by quantitation real time polymerase chain reaction.Immunohistochemistry method was used to detect monocyte chemoattractant protein-1（MCP-1） expressions in renal.Results ：（1）At 29 weeks,the level of MCP-1 in blood serum in G2（827.70±259.36 pg/mL） was more significantly decreased than in the control group（1325.45±352.28 pg/mL）（P〈0.05）,and at 28 weeks,the level of MCP-1 in urine in G2（239.93±31.47 pg/mL） was also more significantly decreased than in the control group（483.52 ±184.37 pg/mL）（P〈0.01）.（2）The degrees of MCP-1 protein in G1（0.40±0.02） and in G2（0.30±0.02） renal were both more significantly decreased than in the control group（0.67±0.05）（P〈0.05） and there is also a significant difference between G1 and G2（P 〈0.05）.（3） The levels of MCP-1 mRNA in G1（0.39±0.02） and in G2（0.30±0.01） renal were both more significantly decreased than in the control group（0.79±0.15）（P〈0.05）.and there is a significantly difference between G1 and G2（P〈0.05）.（4） In immunohistochemical staining kidney sections,MCP-1 was mainly located on glomerular mesangium and renal interstitium and renal tubule and inflammatory cell infiltrate area.The expression MCP-1 of G1 and G2 was more decreased than of the control group.Conclusion：Inhibition of MCP-1 may be one of the mechanisms of UC-MSCs therapeutic effect on MRL/lpr mice.