| 模拟微重力条件下成骨生长肽促进MC3T3-E1成骨样细胞的增殖与分化 |
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| 引用本文： | 刘俊丽,牛忠英,宋淑军,司少艳,周金莲,赵刚,谭小青,张建中.模拟微重力条件下成骨生长肽促进MC3T3-E1成骨样细胞的增殖与分化[J].航天医学与医学工程,2012,25(3):168-171. |
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| 作者姓名： | 刘俊丽 牛忠英 宋淑军 司少艳 周金莲 赵刚 谭小青 张建中 |
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| 作者单位： | 解放军第306医院病理实验科,北京,100101 |
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| 基金项目： | 总装后勤部课题资助项目 |
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| 摘 要： | 目的研究回转模拟微重力(simulated microgravity,SMG)条件下成骨生长肽(OGP10-14)对MC3T3-E1成骨样细胞增殖与分化的影响。方法利用回转器模拟失重,MTT法检测OGP10-14对回转24 h,48h,72 h MC3T3-E1细胞增殖的效应,并观察培养液中碱性磷酸酶(alkaline phosphatase,ALP)、骨桥素(osteopontin,OPN)活性变化。结果回转模拟微重力条件下,10-8mol/L OGP10-14能够显著促进成骨细胞系MC3T3-E1的增殖(P<0.01)并促进成骨细胞系MC3T3-E1的ALP和OPN活性。结论回转模拟微重力条件下,OGP10-14对成骨细胞系MC3T3-E1的增殖与分化具有促进作用。
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| 关 键 词： | 模拟微重力 回转器 成骨细胞 成骨生长肽 增殖 分化 |
Osteogenic Growth Peptide Promotes Differentiation and Proliferation of MC3T3-E1 Osteoblasts under Simulated Microgravity |
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| Liu Junli
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Niu Zhongying
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Song Shujun
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Si Shaoyan
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Zhou Jinlian
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Zhao Gang
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Tan Xiaoqing
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Zhang Jianzhong.Osteogenic Growth Peptide Promotes Differentiation and Proliferation of MC3T3-E1 Osteoblasts under Simulated Microgravity[J].Space Medicine & Medical Engineering,2012,25(3):168-171. |
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| Authors: | Liu Junli Niu Zhongying Song Shujun Si Shaoyan Zhou Jinlian Zhao Gang Tan Xiaoqing Zhang Jianzhong |
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| Institution: | ..Department of Pathology and Experimental Medicine,306 Hospital of PLA,Beijing 100101,China |
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| Abstract: | Objective To study the effect of osteogenic growth peptide(OGP10-14) on the differentiation and proliferation of osteoblast MC3T3-E1 under the conditions of simulated microgravity(SMG).Methods Under SMG 24 h,48 h and 72 h,the effectiveness of the proliferation and differentiation of osteoblasts MC3T3-E1 were examined by MTT.Activity changes of ALP and OPN in the cell culture medium was observed after treated with OGP10-14.Results Under SMG,10-8 mol/L OGP10-14 were able to significantly promote the proliferation of osteoblasts MC3T3-E1(P<0.01) and enhanced the ALP and OPN activity of osteoblasts MC3T3-E1.Conclusion Under SMG,OGP10-14 facilitates the proliferation and differentiation of osteoblasts MC3T3-E1. |
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| Keywords: | simulated microgravity clinostat osteoblasts osteogenic growth peptide proliferation differentiation |
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