回转器模拟微重力对白色念珠菌致病性的影响

目的观察回转器模拟微重力对白色念珠菌致病性的影响。方法回转器处理白色念珠菌SC5314,观察模拟微重力对菌株生长、小鼠的致死能力、小鼠肾脏和大脑的菌落形成、巨噬细胞破坏作用的影响。检测cAMP分子在回转模拟微重力过程中的作用及Gβ-Gα-AC-cAMP信号通路蛋白的基因表达情况。结果回转模拟微重力导致白色念珠菌生长显著加快;对小鼠的致死速度加快;诱导巨噬细胞凋亡的作用显著增强。模拟微重力导致菌株细胞cAMP的水平显著升高;外源性cAMP显著促进菌丝生成,增加其对小鼠的致死率;模拟微重力导致Gβ基因表达显著增加,而其它与cAMP产生的相关基因没有明显变化。结论回转器模拟微重力显著增加白色念珠菌致病性,可能是通过激活Gβ-Gα-AC-cAMP信号转导途径实现的。

Effects of Simulated Microgravity with Clinostat on Pathogenicity of Candida Albicans

Objective To observe the effects of simulated microgravity with clinostat on the pathogenicity of Candida albicans.Methods Candida albicans SC5314 was pretreated with rotating clinostat.Effects of simulated microgravity on the growth curves,mycelium growth rate,lethal effect on mice,colony-forming units(cfu) of brain and kidneys organization of mice and effect on macrophage were observed.In addition,biological effects on treated group were obtained by comparing with the control group including cellular cAMP levels,the expression of genes associated with the protein of Gβ-Gα-AC-cAMP signal pathway.Results Simulated microgravity significantly accelerated the growth and mycelium growth of Candida albicans SC5314.Lethal speed of the test strains on mice was faster than that of the control strains and the influence of the test strains on macrophage apoptosis was more significant than that of the control.Compared with control strains,cAMP levels increased significantly in Candida albicans cells treated with clinostat.Exogenous cAMP significantly enhanced Candida albicans hyphae formation and cAMP-treated Candida albicans increased the mortality in mice.Furthermore,simulated microgravity increased Gβ gene expression in Candida albicans.While,other genes associated with the cAMP were not changed in the expression.Conclusion Simulated microgravity significantly increased the pathogenicity of Candida albicans and the mechanism might be Gβ-Gα-AC-cAMP signal transduction pathway.