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| 转化生长因子受体Ⅱ与去神经性肌萎缩的相关性研究 | |
| 引用本文： | 王旭,王璐,刘红菊,李莉,李景龙,范明,陈晓萍.转化生长因子受体Ⅱ与去神经性肌萎缩的相关性研究[J].航天医学与医学工程,2012,25(3):217-219. |
| 作者姓名： | 王旭 王璐 刘红菊 李莉 李景龙 范明 陈晓萍 |
| 作者单位： | 1. 军事医学科学院基础医学研究所,北京100850;沈阳军区总医院医学实验科,辽宁沈阳110840 2. 中国航天员科研训练中心,北京,100094 3. 军事医学科学院基础医学研究所,北京,100850 |
| 基金项目： | 国家重点基础研究973项目，国家自然科学基金面上项目 |
| 摘 要： | 目的研究去神经致肌萎缩和纤维类型转化的分子机制,探讨TβRII-SMAD信号通路在此过程中的相关性。方法取10只C57BL/6小鼠,右后肢坐骨神经离断,左后肢假手术对照。Real Time PCR(RT-PCR)检测术后14 d比目鱼肌和跖肌萎缩基因MAFbx,MuRF-1,及PGC-1α,MHCIIb,TβRII的表达变化。结果去坐骨神经后萎缩基因MAFbx,MuRF-1及MHCIIb在比目鱼肌和跖肌明显上调;PGC-1α,TβRII的表达在比目鱼肌和跖肌呈现差异性变化。结论 MAFbx,MuRF-1和PGC-1α在肌萎缩过程中发挥重要作用,TβRII可能参与了对肌萎缩的调节。 |
| 关 键 词： | 去神经 肌萎缩 TβRII 分子机制 |
Correlation between TβRⅡ and Denervation-induced Skeletal Muscle Atrophy |
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| Wang Xu , Wang Lu , Liu Hongju , Li Li , Li Jinglong , Fan Ming , Chen Xiaoping.Correlation between TβRⅡ and Denervation-induced Skeletal Muscle Atrophy[J].Space Medicine & Medical Engineering,2012,25(3):217-219. | |
| Authors: | Wang Xu Wang Lu Liu Hongju Li Li Li Jinglong Fan Ming Chen Xiaoping |
| Institution: | .China Astronaut Research and Training Center,Beijing 100094,China;Fan Ming.Institute of Basic Medical Sciences,the Academy of the Military Medical Science,Beijing 100850,China |
| Abstract: | Objective To research the mechanisms of the denervation-induced muscle atrophy,fiber-type switching,and the correlation between TβRII-SMAD and its mechanisms.Methods Sciatic nerves were cut off by 5 mm in the right hind legs of 10 C57BL/6 mice with the sham on the left.QRT-PCR was performed 14 d later for quantification of mRNA expression of genes: MAFbx,MuRF-1,PGC-1α,MHCIIb,and TβRII.Results Relative mRNA levels of MAFbx,MuRF-1,MHCIIb in denervated Sol and Plan muscles were significantly increased and the change of the genes,PGC-1α and TβRII,were significant in Sol and Plan muscles when compared with those in sham-operated contralateral muscles.Conclusion Genes MAFbx,MuRF-1 and PGC-1α play important roles in muscular atrophy,and the gene TβRII may be involved in the adjustment course of it. |
| Keywords: | denervation muscle atrophy TβRII molecular mechanisms |
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