胚胎大鼠纹状体区神经干细胞的体外培养

目的探讨胚胎大鼠纹状体区神经干细胞的培养、分化和鉴定技术。方法从胚胎大鼠纹状体区分离神经干细胞，用无血清培养技术进行体外原代培养、传代，加血清进行诱导分化。荧光免疫染色技术进行鉴定。结果从胚胎大鼠纹状体区分离出神经干细胞，并可分化为神经元、星形胶质细胞和少突胶质细胞。结论采用该体外培养技术分离的纹状体区神经干细胞可在体外大量增殖，并有多向分化潜能。

Cultivation of embryonic striatum-neural stem cells from rat in vitro

Objective To explore the cultivation, differentiation and indentification of embryonic striation-neural stem cells from rat. Methods The advantage of serum free and clone culturing technology was performed to isolate, cultere, passage and induce neural stem cells from embryonic rat cortex, hippocampus and striatum. Immunofluorescence was applied to identify the neural stem cells and there potential of differentiation. Results The cultivated cells had the ability to proliferate continuously and cultivation differentiated into neurons, astrocytes and oligodendrocytes. Conclusion The technique of the separation of in vitro neural stem cells in the striatum may proliferate a large number in vitro and have more differentiation potential.