葛根素对兔脊髓缺血再灌注损伤的保护作用

目的：研究葛根素对兔脊髓缺血再灌注损伤的作用。方法：20只雄性新西兰大白兔随机分成缺血组（A组，n=10）及葛根素组（B组，n=10),夹闭腹主动脉肾下段20min，建立兔脊髓腰尾段血模型，B组于夹闭前10min静脉注射葛根素30mg.kg^-1，A组则静脉注射生理盐水，测定夹闭前、后及再灌注后血浆中丙二醛（MDA）浓度及超氧化物歧化酶（SOD)活性，再灌注4，8，12，24及48h分别对动物后肢运动功能评分；再灌注48h后，处死动物，制作切片，观察其组织病理变化。结果缺血及再灌注后B组MDA值明显低于A组（P＜0．05），而OD活性明显高于A组（P＜0．05）；再灌注后4，8，12，24及48h时的神经功能评分B组明显高于A组（P＜0．05）；光镜下，与A组相比，B组脊髓组织损伤显著减轻，形态基本正常的前角细胞较多。结论葛根素对兔脊髓缺血再灌注损伤有显著的保护作用。

Protective effect of puerarin on spinal cord injury resulting from isch emia and reperfusion in rabbits

AIM　To study the effects of puerarin on spinal cord injury resulting from ischemia and reperfusion. METHODS　Twenty male New Zealands white rabbits were randomly divided into two groups: ischemia group (Group A, n=10) and puerarin group (Group B , n=10). The spinal cord ischemia rabbit model was made by occluding infrarenal abdominal aor ta for 20 min. In Group B, puerarin 30 mg*kg-1 was administered intravenously 10 min be fore clamping. In Group A, 9 g*L-1 saline was used as Group B. The productions of MDA and the activi ties of SOD before,during and after cross-clamping were assayed. Neurologic status was scored at 4,8,12,2 4,48 hours after reperfusion. All animal were killed 48 h after reperfusion, the spinal cords wer e studied histopathologically. RESULTS　During and after cross-clamping , the productions of MDA in Group B were lower than those in Group A (P<0.05), but the activities of SOD in Group B were higher than those in Group A (P<0.05). Neurologic function scores at 4, 8, 12, 24, 48 h after reperfusion were higher in Group B than those in Group A (P<0.05); Compa red to Group A, less degree of injury was seen in Group B under optical microscope 48 h after reperfu sion. CONCLUSION　Puerarin could protect rabbit spinal cord inju ry resulting from ischemia and reperfusion.