人参多糖与人参皂苷诱导大鼠骨髓间充质干细胞造血细胞因子表达的作用比较

目的 了解骨髓多能间充质干细胞(BM-MSC) 主要造血因子mRNA表达水平，比较人参多糖和人参皂苷对BM-MSC造血因子mRNA表达的影响。 方法 采用Real-time PCR技术，观察人参多糖(20 μg/mL)或人参皂苷(20 μg/mL)作用于大鼠BM-MSC 12、24、36 h时间点造血因子IL4、Csf2、Kitlg、Csf1、IL6、Lif、Csf3、IL11、Epo和IL3等10个基因mRNA相对表达水平。 结果 正常大鼠BM-MSC未检测到IL4和Csf2 mRNA表达，以内参基因Gapdh mRNA作参比, Kitlg、Csf1、IL6、Lif、Csf3、IL11、Epo和IL3等mRNA相对表达量依次减弱。Epo和IL3 mRNA表达在人参多糖与人参皂苷作用后各个时间点均无明显影响(P>0.05)。人参多糖在12 h和36 h时间点对BM-MSC的Csf1、IL6、Lif、Csf3和IL11等5个造血因子mRNA表达有显著促进作用(P<0.05)，在24 h时间点对Csf1、IL6、Lif、Csf3和Kitlg等5个造血因子mRNA表达有显著促进作用(P<0.05)。人参皂苷在12、24和36 h时间点对BM-MSC有促进表达的基因分别为1个(Csf3)，3个(Csf3、IL6和Lif)，5个(Csf3、IL6、Lif、IL11和Kitlg)。

Comparative Study of Enhancing Effect on mRNA Expression of Hematopoietic Growth Factors in Rat Bone Marrow Mesenchymal Stem Cells by Ginseng Polysaccharide and Ginsenoside

Objective To study mRNA expression levels of main hematopoietic growth factors in bone marrow mesenchymal stem cells（BM-MSC）,and to compare effect on mRNA expression levels treated by ginseng polysaccharide and ginsenoside.Methods Relative quantification Real-time polymerase chain reaction（RT-PCR） was used to observe mRNA expression levels of IL4,Csf2,Kitlg,Csf1,IL6,Lif,Csf3,IL11,Epo,and IL3,etc.in rat BM-MSC treated with ginseng polysaccharide（20 μg/mL） or ginsenoside（20 μg/mL） at 12,24,and 36 h.Results IL4 and Csf2 mRNA expressions were not detected.Relative expression of Kitlg,Csf1,IL6,Lif,Csf3,IL11,Epo and IL3 mRNA ranked in an attenuating order when compared with Gapdh mRNA.mRNA expression of Epo and IL3 was not significantly changed at any time point by treatment of ginseng polysaccharide or ginsenoside in rat BM-MSC（P0.05）.mRNA expression of Csf1,IL6,Lif,Csf3 and IL11 were significantly enhanced at 12 and 36 h by treatment of ginseng polysaccharide（P0.05） and that of Csf1,IL6,Lif,Csf3,and Kitlg were significantly enhanced at 24 h in rat BM-MSC（P0.05）.The enhanced mRNA expression was Csf3 at 12 h,Csf3,IL6 and Lif at 24 h,and Csf3,IL6,Lif,IL11,and Kitlg,respectively at 36 h by treatment of ginsenoside in rat BM-MSC.Conclusions The enhancement of ginseng polysaccharide was stronger than that of ginsenoside on mRNA expression of hematopoietic growth factors in the initial stage.As time went by,the enhancement of ginsenoside gradually increased and exceeded that of ginseng polysaccharide.