| 淋病奈瑟氏菌L型分子生物学性状的研究 |
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| 引用本文： | 程兴望,唐宁枫,林特夫,黄谷良. 淋病奈瑟氏菌L型分子生物学性状的研究[J]. 皮肤病与性病, 2001, 23(2): 5-6 |
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| 作者姓名： | 程兴望 唐宁枫 林特夫 黄谷良 |
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| 作者单位： | 1. 安徽省蚌埠医学院附属医院急诊外科,安徽,蚌埠,233000
2. 蚌埠医学院微生物教研室 |
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| 摘 要： | 细菌变成Ｌ型后 ,许多生物学性状与原菌不同。为了解淋病奈瑟氏菌 (Ｎｅｉｓｓｅｒｉａｇｏｎｏｒｒｈｏｅａｅ ,ＮＧ)由细菌型变为Ｌ型后 ,随着形态学的改变 ,其蛋白组成和免疫学特征有何变化 ,我们对ＮＧ细菌型和Ｌ型做了十二烷基磺酸钠———聚丙酰胺凝胶电泳 (ＳＤＳ—ＰＡＧＥ)和用兔抗ＮＧ血清做了免疫印迹分析 (ｗｅｓｔｅｒｎ -ｂｌｏｔ)。1 材料与方法1 1 材料1 1 1 实验菌株细菌型 :ＮＧ标准菌株 2 410 9购自卫生部北京药品生物制品鉴定所及 7株自病人分离的ＮＧ。Ｌ型 :用苯唑青霉素诱导ＮＧ2 410 9株成缺壁菌 ,并在含抗生…
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| 关 键 词： | 淋病奈瑟氏菌L型 分子生物学性状 NG抗体 免疫印迹 |
| 文章编号： | 1002-1310(2001)02-0005-02 |
| 修稿时间： | 2000-02-15 |
The study of some molecular characteristics N.gonorrhoeae L-form |
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| CHENG Xing-wang,TANG Ning-feng,LIN Te-fu,et al. The study of some molecular characteristics N.gonorrhoeae L-form[J]. Journal of Dermatology and Venereology, 2001, 23(2): 5-6 |
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| Authors: | CHENG Xing-wang TANG Ning-feng LIN Te-fu et al |
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| Abstract: | In order to analyze the protein composition of N.gonorrhoeae and their 1-forms,SDS-PACE and western-bolt were performed.In SDS-PACE,2 protein bands(66 2 and 14 4 KD)lost and 2 bands(34 and 24 KD)decreased in density.In western blot with the using of anti-N.gonorrhoeae serum,the major outer membrane protein(between 21 and 37 KD)still existed in both N.gonorrhoeae and its L-form,but the decrease in density and 2 additional bands of N.gonorrhoeae were visible in L-form,The electrophoretic pattern in L-forms of N.gonorrhoeae,however,were basically unchanged.Therefore,it can still be used for indentification of N.gonorrhoeae L-form |
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| Keywords: | N.gonorrhoeae laboratory diagnosis |
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