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| 新生血管特异抗体的制备及生物活性鉴定 | |
| 引用本文： | 阎锡蕴,杨东玲,沈毅,林芸,袁玫,李力,夏红天,李沛雨,刘琴,罗丹丹.新生血管特异抗体的制备及生物活性鉴定[J].中华微生物学和免疫学杂志,2003,23(8):642-645. |
| 作者姓名： | 阎锡蕴 杨东玲 沈毅 林芸 袁玫 李力 夏红天 李沛雨 刘琴 罗丹丹 |
| 作者单位： | 1. 中国科学院生物物理所 2. 中国科学院微生物所 3. 解放军总医院 |
| 基金项目： | 国家 8 63 (2 0 0 1AA2 15 3 2 1)，国家自然科学基金(C0 3 0 3 190 6)资助项目，中国科学院创新项目 (KSCX2 SW 2 13 ) |
| 摘 要： | 目的 研制识别肿瘤新生血管的单克隆抗体及其活性。方法 用肝癌细胞条件培养基刺激人血管内皮细胞，以此作为抗原免疫小鼠制备抗体。用免疫组化、ELISA和流式细胞术筛选肿瘤血管特异抗体并鉴定其免疫特性。用MTT法研究抗体对血管内皮细胞增殖的影响。结果 抗体AA98选择性地识别肿瘤血管和人血管内皮细胞，抑制血管内皮细胞的增殖。结论 抗体AA98在肿瘤诊断和治疗方面具有潜在的应用价值。 |
| 关 键 词： | 肿瘤 血管导向治疗 新生血管 单克隆抗体 生物活性 |
| 修稿时间： | 2003年1月15日 |
A novel monoclonal antibody to tumor neovasculature inhibits the proliferation of human vascular endothelial cells |
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| YAN Xi yun ,YANG Dong ling,SHEN Yi,LIN Yun,YUAN Mei,LI Li,XIA Hong tian,LI Pei yu,LIU Qin,LUO Dan dan. Biophysics Institute,Chinese Academy of Sciences,Beijing ,China.A novel monoclonal antibody to tumor neovasculature inhibits the proliferation of human vascular endothelial cells[J].Chinese Journal of Microbiology and Immunology,2003,23(8):642-645. | |
| Authors: | YAN Xi yun YANG Dong ling SHEN Yi LIN Yun YUAN Mei LI Li XIA Hong tian LI Pei yu LIU Qin LUO Dan dan Biophysics Institute Chinese Academy of Sciences Beijing China |
| Institution: | YAN Xi yun *,YANG Dong ling,SHEN Yi,LIN Yun,YUAN Mei,LI Li,XIA Hong tian,LI Pei yu,LIU Qin,LUO Dan dan. *Biophysics Institute,Chinese Academy of Sciences,Beijing 100101,China |
| Abstract: | Objective To develop an antibody which recognizes a novel target on tumor neovasculature. Methods Monoclonal antibodies were prepared with the mice that immunized by human umbilical vascular endothelial cells (HUVEC). The specificity of the antibodies was characterized with immunohistochemistry,ELISA and FACS analysis. The effect of antibody AA98 on the proliferation of HUVEC was analyzed by MTT method. Results Antibody AA98 selectively bound to tumor neovasculatures and angiogenic HUVECs. The antibody inhibited proliferation of endothelial cells. Conclusion The antibody AA98 has a potential application for tumor diagnosis and therapy. |
| Keywords: | Angiogenesis Monoclonal antibody Tumor |
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