| 1，25双羟维生素D3对成骨样细胞骨架和基因表达的影响 |
| |
| 引用本文： | 薛延,袁润英,相东. 1，25双羟维生素D3对成骨样细胞骨架和基因表达的影响[J]. 解剖学报, 1998, 29(1): 67-71,I012 |
| |
| 作者姓名： | 薛延 袁润英 相东 |
| |
| 作者单位： | [1]北京积水潭医院生化研究室 [2]北京市神经外科研究所细胞生物学研究室 |
| |
| 摘 要： | 用荧光免疫法研究了１，２５双羟基维生素Ｄ３对大鼠成骨样细胞（ＲＯＳ１７／２．８）细胞骨架的影响和用斑点杂交技术研究了１，２５（ＯＨ）２Ｄ３对人成骨样细胞（ＯＳ－７３２）基因表达的影响。结果表明：在１０＾－７ｍｏｌ／Ｌ１，２５（ＯＨ）２Ｄ３连续作用４ｄ后，大鼠ＲＯＳ１７／２．８细胞的微管蛋白和波形纤维蛋白明显改善，纤粘蛋白荧光明显增强。而在１，２５（ＯＨ）２Ｄ３作用前后ＯＳ－７３２细胞ｃ－ｍｙｃ和ｍ
|
| 关 键 词： | 双羟基维生素D3 成骨样细胞 细胞骨架 基因表达 |
EFFECT OF 1,25 DIHYDROXYVITAMIN D 3 ON CYTOSKELTAL STRUCTURE AND GENE EXPRESSION IN OSTEOBLAST LIKE CELLS |
| |
| Abstract: | The study was carried out to investigate the effects of 1,25 dihydroxyvitamin D 3 1, 25(OH) 2D 3 on cytoskeleton in rat osteoblast like cells (ROS 17/2.8) by immunofluorescent staining methods and on gene expression in human osteoblast like cells (OS 732) by a dot blot techique. The results showed that the microtubules and vimentins improved and the immunoflurorescence of fibronectins increased in ROS 17/2.8 cells after 4 days using 10 -7 mol/L 1,25 (OH) 2D 3 treatment. However c myc and met D highly expressed in OS 732 cells after and before 1,25(OH) 2D 3 treatment. Also the relationships of cell differentiation and cytoskeletal structure and gene expression were discussed. |
| |
| Keywords: | 1 25 Dihydroxyvitamin D 3 Osteoblast like cell Cytoskeleton Fibronectin Gene expression |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
