大鼠下丘脑内神经激肽B受体阳性神经元向孤束核的直接投射

为了研究神经激可能的升压途径，用免疫荧光组织化学方法结合荧光金逆行追踪技术 下丘脑内神经激肽Ｂ受体阳性神经元向孤束核的投射。

NEUROKININ B RECEPTOR (NKR) POSITIVE NEURONS IN THE HYPOTHALAMUS PROJECTING TO THE NUCLEUS OF THE SOLITARY TRACT IN THE RAT

Direct projection of the neurons expressing neurokinin B receptor(NKR) from the hypothalamus to the nucleus of the solitary tract(NTS) was carried out in the rat by using fluorescence immunohistochemistry combined with fluorogold(FG) retrograde tracing technique.After injection of FG into the NTS,FG labeled neurons were seen in the dorsal hypothalamic area,paraventricular nucleus,perifornical area,dorsal and ventrolateral parts of the lateral hypothalamic area.On the other hand,NKR positive neurons were mainly observed in the dorsal hypothalamic area,paraventricular nucleus,perifornical area,medial part of the lateral hypothalamic area,supraoptic nucleus and medial part of the medial mammillary nucleus.Immunofluoresent staining showed that FG/NKR double labeled neurons were found in the dorsal hypothalamic area,perifornical area,dorsal and ventrolateral parts of the lateral hypothalamus area.Most of them were medium sized,and round or oval in shape.The doubly labeled neurons constituted 11 2% of the FG labeled neurons and 11 9% of the NKR positive neurons,respectively.The present results revealed that some neurons expressing NKR in the hypothalamus projected to the NTS and suggest that they might be involved in the regulation of blood pressure.