多参数流式细胞术对外周血白血病免疫分型的研究

目的 :探讨多参数流式细胞术、CD45/SCC设门技术和三色荧光标记抗体 ,检测外周血在白血病免疫分型中的临床应用及其意义。方法 :对检测标本以CD45- Percp/SSC设门技术及流式细胞仪多参数分析技术 ,采用单克隆抗体三色标记对白血病患者的细胞群体进行免疫表型分析。结果 :195例白血病患者 ,其中急性白血病 14 4例 ,慢性白血病 51例。 14 4例急性白血病患者中 ,13 7例出现可供分析的白血病细胞独立群体。免疫分型结果ALL 66例 ,AML 67例 ,未能分型 4例。 2 7例AL表达 2个不同系列的免疫标记 (2 0 % )。分析了各类白血病的免疫表型的特征 ,AL共表达不同系列的抗原是一种常见现象。结论 :利用CD45 Percp/SSC设门技术及流式细胞仪多参数分析技术 ,可对白血病细胞群体进行准确的免疫表型检测和研究 ,有助于指导临床治疗及判断预后

Immunophenotyping of peripheral blood leukemic cells by multi-parameter cytometry

AIM: To explore the clinical application and significance of CD45/SCC gating strategy of multi-parameter cytometry and tricolor fluorescence-labele d monoclonal antibodies in immunophenotyping of peripheral blood leukocytes in l eukemia. METHODS: Peripheral blood leukocytes were stained with CD45 combined with a panel of monoclonal antibodies to different lineage marker s. CD45/SSC dot plot was formed and leukemic cell colony, once displayed, was ga ted for immunophenotyping. RESULTS: 144 patients with acute leukemia and 51 with chronic leukemia were enrolled. Distinguishable leukemic c ell colony was found in 137 out of 144 specimens. 66 patients were immunologica lly classified as ALL and 67 as AML. 4 patients were unable to be classified imm unophenotypically. 27 AL patients coexpressed markers of another cell lineage (2 0%). Immunophenotypic features of different types of leukemia were described. It is a commen phenomenon that AL of one lineage coexpressed markers of another li neage. CONCLUSION: An accurate immunophenotyping of leukemia ca n be realised when leukemic cell colony is displayed and analyzed by CD45/SCC p attern and gating strategy, which is important for clinical treatment and progno sis judgment.