| 高效毛细管电泳法测定栀子中栀子苷的含量 |
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| 引用本文： | 王洪平,周洪雷,李和光,窦月. 高效毛细管电泳法测定栀子中栀子苷的含量[J]. 辽宁中医杂志, 2010, 0(2): 318-319 |
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| 作者姓名： | 王洪平 周洪雷 李和光 窦月 |
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| 作者单位： | 山东中医药大学; |
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| 基金项目： | 国家重点基础研究发展计划(973计划)资助项目(2007CB512601) |
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| 摘 要： | 目的:测定中药栀子中栀子苷的含量。方法:采用高效毛细管电泳法,电泳条件为石英毛细管柱(50μm×83cm,实际长度74cm),运行缓冲液为0.05mol.L-1Na2B4O7:0.01mol.L-1Na2HPO4=5∶1(16℃pH=9.2),分离电压为28kV,压力进样30mbar×8s,温度为25℃,检测波长为238nm。结果:栀子苷在0.5～1.75mg.mL-1范围内呈良好的线性关系(r=0.9998),平均回收率为99.64%,RSD为1.23%。结论:高效毛细管电泳法可用于测定栀子中栀子苷的含量。
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| 关 键 词： | 栀子 栀子苷 高效毛细管电泳法 |
Determination of Geniposide in Fructus Gardeniae by High Performance Capillary Electrophorsis |
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| WANG Hong-ping,ZHOU Hong-lei,LI He-guang,DOU Yue. Determination of Geniposide in Fructus Gardeniae by High Performance Capillary Electrophorsis[J]. Liaoning Journal of Traditional Chinese Medicine, 2010, 0(2): 318-319 |
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| Authors: | WANG Hong-ping ZHOU Hong-lei LI He-guang DOU Yue |
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| Affiliation: | Shandong University of TCM/a>;Jinan 250355/a>;Shandong/a>;China |
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| Abstract: | Objective：To determine the content of Geniposide Baicalin in Fructus gardeniae.Methods：Apply the methods of HPCE,and the best condition was using the quartz capillary tube（50μm×83cm,practical length is74cm）,0.05mol·L-1Na_2B_4O_7：0.01mol·L-1Na_2HPO_4=5∶1（pH=9.2 at 16℃）buffer,pressure injection 30mbar×8s,at a constant voltage of 28kV and temperature at 25℃.It was detected on 238nm.Results：Geniposide Baicalin had a good linearity within 0.5～1.75mg·mL-1（r=0.9998）,and the average recovery were 99.64% with RSD 1.23%.Conclusion：This method is feasible for determination of Geniposide Baicalin in Fructus gardeniae. |
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| Keywords: | fructus gardeniae Geniposide HPCE |
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