藿香和广藿香挥发油对皮肤癣菌和条件致病真菌的抑制作用

 目的：筛选天然抗皮肤真菌新药和开发芳香油植物资源。方法目的：筛选天然抗皮肤真菌新药和开发芳香油植物资源。方法：利用培养基药物浓度稀释法和气相色谱质谱联用技术，研究了藿香和广藿香挥发油对12种皮肤癣菌和条件致病真菌的体外抑制作用和主要化学成分，比较了3种不同地理来源（中国、印度和印度尼西亚爪哇）广藿香油的抗真菌活性。结果：这4种挥发油都可以选择性地完全抑制皮肤癣菌的生长繁殖，其中中国广藿香油的活性最强，其最低抑制浓度（MIC）50～400 μl·L^-1，主要化学成分为广藿香醇、异愈创木烯和广藿香烯。藿香油最弱，MIC位于700～1000 μl·L^-1，主要成分是薄荷酮类化合物。结论：证明了广藿香油的选择性皮肤癣菌抑制作用，为进一步筛选抗真菌外用新药提供了重要依据。：利用培养基药物浓度稀释法和气相色谱一质语联用技术，研究了蓄香和广曹香挥发油时12种皮肤癣菌和条件致病真菌的体外抑制作用和主要化学成分，比较了3种不同地理来源(中国、印度和印度尼西亚爪哇)广茬香油的杭真菌活性。结果：这4种挥发油都可以选择性地完全抑制皮肤癣菌的生长繁殖，其中中国广着香油的活性最强，其最低抑制浓度(MIC)50～400μl·L/〈sup〉-1〈sup〉，主要化学成分为广蓉香醇、异愈创木烯和广蓉香烯。蓉香油最弱，MIC位于700～1000μl·Ｌ〈sup〉-1〈sup〉，主要成分是薄荷酮类化合物。结论：证明了广茬香油的选择性皮肤癣菌抑制作用，为进一步筛选杭真菌外用新药提供了重要依据。

Antifungal activity of the essential oils from Agastache rugosa and Pogostemon cablin against dermatophytes and opportunistic fungi

OBJECTIVE：To search some new natural skin antifung al drugs and to exploit the aromatic plant resources. METHOD：The antifungal screening and the major chemical ingre dients of the essential oils extracted from the leaves of Agastache rugosa a nd Pogostemon cablin (patchouli) were studied by media dilution against twel ve dermatophytes and opportunistic fungi and by GC-MS technique. The inhibitor y activity of three patchouli oils of various geographical origins (China, India and Indonesia Java) were compared. RESULTS: These oils inhibited totally the growth of dermatoph ytes, but had extremely weak effect against opportunistic fungi in vitro. T he Chinese patchouli oil was the most efficacious with a MIC value of 50～400 μ l.L-1, its principal components were patchouli alcohol,α-bulnesene andpatchoulene. The oil of A. rugosa had a poor antifungal effect with MIC of 700～1000 μl.L-1, it is mainly composed of the menthone deriva tives. CONCLUSION：This study suggested that the patchouli oil had s pecific antifungal effect against dermatophysis with good prospects.