血管紧张素原基因多态性与血管性认知障碍关系的研究

目的 探讨血管紧张素原(AGT)基因G-6A和M235T多态性与血管性认知障碍(VCI)的关系.方法 采用随机对照研究,聚合酶链反应(PCR)方法检测VCI组(67例)、正常对照组(71例)AGT基因G-6A和M235T多态性.结果 VCI组AGT基因M235TT等位基因频率0.73,TT基因型频率0.52,与对照组(0.68,0.45)比较差异无统计学意义(P0.05),TT基因型对VCI的比数比为0.544(95%CI为0.208～1.424,P0.05).VCI组AGT基因G-6 A等位基因频率0.69,AA基因型频率0.48,与对照组(0.63,0.39)比较差异无统计学意义(P0.05),AA基因型对VCI的比数比为0.602(95%CI为0.252～1.738.P0.05).结论 脑梗死伴血管性认知障碍患者与AGT基因G-6A和M235T多态性无关,AGT基因这两个位点多态性未参与发病.

Relationship between angiotensinogen gene polymorphism and vascular cognitive impairment

Objective To investigate the relationship between vascular cognitive impairment(VCI) and the angiotensinogen(AGT) gene ( G-6A and M235T) polymorphism. Methods Randomnized controled study was ap- plied in the study. AGT gene G-6A and M235T genotypes of 67 cases with VCI and 71 normal controls were deter- mined by polymerase chain reaction (PCR). Results For the location of M235T, the frequencies of T allele(0.73 I and TT genotype ( 0.52 ) were observed in VCI compared with control group ( 0.68,0.45, P > 0.05 ). The odds ratio associated with TT/MM genotype was 0.544 ( 95% CI 0.208～1. 424 ,P > 0.05 ). For the location of G-6A ,the fre- quencies of A allele(0.69) and AA genotype (0.48) were observed in VCI compared with control subjects (0.63, 0.39,P > 0.05). The odds ratio associated with AA/GG genotype was 0.602 ( 95% CI 0.252～1. 738, P > 0.05 ). There was no difference in allele distribution between 67 VCI patients and the controls. Conclusion There is no correlation between vascular cognitive impairment and AGT gene polymorphisms of M235T and G-6A. AGT gene pol- ymorphism is not included in the risk factors for development vascular cognitive impairment.