大鼠烫伤后肠黏膜上调蛋白质组的分离鉴定及其功能分析

目的　观察严重烧伤后早期肠黏膜上调蛋白的整体变化特性 ,并对明确的蛋白质进行功能分析 ,为探讨严重烧伤后肠黏膜损害的发病机制提供理论依据。　方法　采用大鼠Ⅲ度烫伤模型 ,利用高分辨双向电泳 ( 2 -DE)对肠黏膜组织进行蛋白质分离 ,ImageMaster 2DElite图像分析软件进行分析 ,应用生物质谱、蛋白质库及文献分析等技术对差异蛋白质中的上调蛋白质组进行鉴定、功能和临床意义分析。　结果　 ( 1)烧伤后 6和 12h两组明确下调的蛋白质点数为34,进行检测及功能分析的蛋白质 2 2个 ;( 2 )糖调控蛋白前体 78、蛋白质二硫化物异构酶A3(PDIA3)前体、基质金属蛋白酶 11、α -烯醇酶和鸟氨酸转化酶等参与了对应激反应的调控。有其他蛋白质 ,如白蛋白、乳酸脱氢酶A、T细胞活化连接蛋白、辅酶Q和eps8捆绑蛋白呈现上调表达。　结论　这些上调的蛋白质的表达改变主要参与了肠黏膜的应激反应及其他病理反应的调控。

Separation and identification of up-regulated proteomics of intestinal mucosa of burnt rats

Objective To investigate the general changes of up regulated proteomics of rat intestinal mucosa early after severe burn injury and analyze the function of the specified proteomics so as to provide theoretical basis for the pathogenesis of damage to intestinal mucosa following severe burn injury. Methods An experimental model of 30% TBSA full thickness scald rat was used for separation of proteomics from intestinal mucosa by using two dimensional electrophoresis (2 DE). ImageMaster 2D Elite, mass spectrometry, protein bank and bibliometrics were applied to identify the up regulated proteomics in the difference proteins and analyze their clinical significance and function. Results (1) At the 6th and 12th hours post burn in both groups, the up regulated proteomics were 34, of which 22 were used for assay and functional analysis. (2) 78 glucose regulated protein precursor (GRP 78), protein disulfide isomerase A3 precursor (PDIA3), matrix metalloproteinase 11 (stromelysin 3), alpha enolase and ornithine aminotransferase participated in the regulation of stress reaction in intestinal mucosa of burnt rats. (3) Up regulated expression appeared in albumin, eps8 binding protein (e3B1), lactate dehydrogenase A and linker for activation of T cells in intestinal mucosa of burnt rats. Conclusion The up regulated proteomics mainly participate in regulation of stress and other pathologic reactions in intestinal mucosa of burnt rats.