| 高效液相色谱法测定燕麦麸皮芦丁的含量 |
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| 引用本文： | 张维库,续洁琨,赵莹,杜冠华. 高效液相色谱法测定燕麦麸皮芦丁的含量[J]. 亚太传统医药, 2010, 6(10): 40-41 |
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| 作者姓名： | 张维库 续洁琨 赵莹 杜冠华 |
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| 作者单位： | 1. 中国医学科学院/北京协和医学院,药物研究所,国家药物筛选中心,北京,100050
2. 北京中医药大学,基础医学院,北京,100029 |
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| 摘 要： | 目的:建立燕麦麸皮中芦丁的含量测定方法。方法:燕麦麸皮经甲醇提取,提取物用甲醇溶解,定容,HPLC分析条件为流动相甲醇-0.4%磷酸(45:55),流速1.0mL/min,柱温30°C,360nm波长下检测样品芦丁的含量。结果:线性方程为Y=0.0007X-0.0029,r=0.9997,线性范围0.025～0.125μg,平均回收率99.58%,RSD为1.32%。结论:本方法简单、快捷,重复性良好,可用于燕麦麸皮中芦丁含量的质量控制。
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| 关 键 词： | 燕麦 燕麦麸皮 芦丁 |
Determination of Lutin in Oat Bran |
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| Zhang Weiku,Xu Jiekun,Zhao Ying,Du Guanhua. Determination of Lutin in Oat Bran[J]. Asia-Pacific Traditional Medicine, 2010, 6(10): 40-41 |
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| Authors: | Zhang Weiku Xu Jiekun Zhao Ying Du Guanhua |
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| Affiliation: | 1.National Center for Pharmaceutical Screening,Institute of Materia Medica,Chinese Academy of Medical Sciences ＆Peking Union Medical College,Beijing 100050,China;2.School of Preclinical Medicine,Beijing University of Chinese Medicine,Beijing 100029,China) |
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| Abstract: | Objective：To develop an HPLC method for determination of Lutin in oat bran.Methods：The methanol extract of oat bran was dissolved by methanol and Lutin was measured by HPLC（mobile phase：CH3OH：0.4%H3PO4=45：55;flow rate：1.0mL/min;temperature：30°C;wave length：360nm） .Results：The calibration curve was in good linearity over the range of 0.025～0.125μg with the regression equation of y=0.0007x-0.0029（r=0.9997） and the average recovery was 99.58%（RSD=1.32%） .Conclusion：The method is simple,quick and reproducible and can be applied in the quality control of oat bran. |
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| Keywords: | Avena Sativa Linn. Oat Bran Lutin |
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