丙酮酸乙酯对严重腹腔感染时肠黏膜过氧化损伤的防治作用

目的探讨丙酮酸乙酯对严重腹腔感染大鼠肠黏膜过氧化损伤的防治作用。方法将36只SD大鼠按照随机数字表法分成对照组、腹腔感染组和治疗组，每组12只。腹腔感染组采用盲肠结扎加穿孔术（CLP）制作严重腹腔感染模型;治疗组行CLP后皮下注射丙酮酸乙酯40mg／kg，每8h1次；对照组仅行单纯剖腹手术。各组于术后24h和48h分别取小肠组织进行病理学观察，记录小肠黏膜病理损伤分级，同时检测小肠及血清丙二醛（MDA）含量、小肠髓过氧化物酶（MP0）活性。结果术后24h和48h腹腔感染组血清和小肠MDA水平均显著高于对照组（P均〈0．05）。且两者呈显著正相关（r=0．867，P〈0．05）；治疗组较腹腔感染组小肠黏膜病理性损害明显减轻，病理分级显著降低（P均〈0．05）；治疗组血清MDA和小肠MPO水平均明显低于腹腔感染组（P均〈0．05）。结论严重腹腔感染时过氧化损伤导致肠黏膜屏障功能障碍，丙酮酸乙酯具有减轻大鼠肠黏膜过氧化损伤的作用。

Effect of ethyl pyruvate on peroxidation injury of intestinal mucosa in rats with severe abdominal infection

Objective To investigate the preventive effect of ethyl pyruvate(EP) on peroxidation injury to intestinal mucosa in rats with severe abdominal infection.Methods Thirtysix SD rats were divided randomly into three groups(n=30 in each group): control group(laparotomy only),infection group(cecal ligation and puncture(CLP) was performed to reproduce severe abdominal infection model) and EP group(CLP plus 40 mg/kg EP subcutaneous injection,once per 8 hours).The changes in intestinal mucosa pathologic score were observed,and malondialdehyde(MDA) and myeloperoxidase(MPO) activities(in intestinal) tissue,and serum MDA levels were determined at postoperative 24 and 48 hours.(Results) (Inflammation) of small intestine mucosa was more severe in the infection group than in EP group, and the pathologic scores were lower in EP group than those of the infection group at postCLP 24 and 48 hours((all P<0.05)).There was a significant positive correlation between the intestinal and plasma MDA in the(infection) group(r=0.867,P<0.05).The MDA and MPO levels in intestinal tissue and serum were(higher) in the infection group than in EP group and control group(all P<0.05).Conclusion With severe intraperitoneal infection in rats,the intestinal mucosa is damaged by the reactive oxygen species.EP could ameliorate the injury of intestinal mucosa by attenuating the injurious effects of the reactive oxygen species.