食管鳞状细胞癌淋巴结转移与血清蛋白指纹图谱的相关性研究

目的 分析食管鳞状细胞癌(ESCC)患者血清蛋白表达谱的改变;比较有无淋巴结转移(LM)的患者血清蛋白指纹图谱,筛选与LM相关的蛋白峰.方法 术前收集64例ESCC患者和60名性别、年龄相匹配的健康成年人的血清.采用弱阳离子交换型(WCX-2)蛋白芯片为检查介质,经SELDI-TOF-MS测定蛋白指纹图谱.行有癌和无癌的对照研究;ESCC患者组内分别按性别、年龄、肿瘤位置、肿瘤大小、浸润程度和有无LM分别进行对照,得到蛋白指纹图谱并用Biomarker Wizard软件分析比较.结果 在质量/电荷(m/z)0～50000范围内,ESCC与健康成年人血清检测出120个蛋白峰,其中31个蛋白峰差异有统计学意义(P<0.05);不同浸润程度分组对比,T1与T3、T4比较有1个m/z是4174的差异蛋白峰(P<0.05);有无LM组间,3个蛋白峰差异有统计学意义(P<0.05),m/z分别为3970、4174、和4277.其中m/z为4174的蛋白峰是浸润组和LM组所共有的;年龄、性别、肿瘤位置、肿瘤大小分组未发现差异蛋白峰.结论 ESCC患者组和健康成年人组比较,血清蛋白有显著差异;ESCC浸润程度的不同和有无LM血清蛋白指纹存在一定差异,但远较ESCC与健康成年人之间差异小;浸润程度严重和LM在血清蛋白指纹中存在着一定的共性.

Relationship between esophageal squamous cell carcinoma with lymph node metastasis and serum proteome fingerprint

Objective To analyze the alterations of serum protein in ESCC,compare alterations of serum protein with and without LM. Methods Serum samples were collected from 64 ESCC patients before operation and 60 cases with gender and age-matched healthy controls,special serum protein or peptide spectra was determined by SELDI-TOF-MS measurement after treating the sample onto weak cation exchange (WCX2) protein chip for each case. The serum protein profiles were compared by Biomarker Wizard Software between the ESCC patients and healthy controls, and among ESCC patients stratified according to gender, age, location of tumor, size of tumor, infiltration and with or without LM. Results (1)120 protein peaks were detected at the molecular range of 0 to 50000 in comparing of ESCC patients and healthy controls. 31 significantly different peaks were found between ESCC patients and healthy controls (P <0.05), 10 peaks were selected(P<0.01). (2) One significantly different protein peak (m/z 4174) was detected between T1 and T3, T4 (P<0.05). (3) There were three significantly different protein peaks (m/z 3970,4174 and 4277) between with LM and without LM (P<0.05).The peak (m/z 4174) was shared by two groups above. (4) No significant different protein was found when patients stratified according to gender, age, location of tumor and size of tumor. Conclusion Significant difference exists in serum proteins between ESCC patients and healthy controls. There are statistical difference exists in serum proteins between T1 and T3, T4, with LM and without LM. This difference is less than between ESCC patients and healthy controls. Some commonness is existed in serum protein fingerprint for patients with serious infiltration and with LM.